The fine-tuning of endoplasmic reticulum stress response and autophagy activation during trophoblast syncytialization

Article Article scientifique Article The fine-tuning of endoplasmic reticulum stress response and autophagy activation during trophoblast syncytialization eng 2019 author Bastida Ruiz Daniel 759554 author Yart Lucile Adeline 862030 author Wuillemin-Rambosson Christine Gabrielle 119160 author Ribaux Pascale Garlonne 543255 author Morris Nolwenn 884529 author Epiney Emmanuela 850809 author Martinez De Tejada Weber Begona 590919 author Cohen Marie-Benoîte 0000-0003-4621-5464 135615 651 10.1038/s41419-019-1905-6 31501418 2041-4889 http://www.nature.com/articles/s41419-019-1905-6 The syncytiotrophoblast (STB) is a multinuclear layer forming the outer surface of the fetal part of the placenta deriving from villous cytotrophoblastic cell (vCTB) fusion and differentiation. This syncytialization process is characterized by morphological and biochemical alterations of the trophoblast, which probably require removal of pre-existing structures and proteins to maintain cell homeostasis and survival. Interestingly, autophagy, which allows degradation and recycling of cellular components, was shown to be activated in syncytiotrophoblast. Here we examined the involvement of endoplasmic reticulum stress (ERS) response in autophagy activation during vCTB syncytialization. We first demonstrated the activation of ERS response and autophagy during the time course of trophoblastic cell fusion and differentiation. Alteration of autophagy activation in vCTB by chemical treatments or Beclin-1 expression modulation leads to a decrease in trophoblastic syncytialization. Furthermore, ERS response inhibition by chemical treatment or siRNA strategy leads to a default in syncytialization, associated with alteration of autophagy markers and cell survival. From these data, we suggest that ERS response, by fine regulation of autophagy activation, may serve as an adaptive mechanism to promote cell survival during trophoblastic syncytialization. Dewey 618 Swiss National Science Foundation Role of GRP78 in cell-cell fusion 163395 CH Project funding 58 Département de pédiatrie, gynécologie et obstétrique 223 false 584 Laboratoire de biologie des tumeurs gynécologiques et du développement 927 213 Martinez De Tejada Begona 272 Cell Death & Disease 10 9 c0409185-45d8-4fc8-a719-b509b56a4fe4 Article (Published version) Public celldeathdis.pdf 2269096 application/pdf CC BY e1f4bfd4-52da-4f73-a6a1-bb9305ee6d9e Supplemental data Public 41419_2019_1905_MOESM1_ESM.pdf 126184 application/pdf 14973bd3-7e79-4c18-ab1e-4cd1b45879ec Supplemental data Public 41419_2019_1905_MOESM2_ESM.pdf 332719 application/pdf 7bf1843d-52bc-44bf-9344-011cbb281d27 Supplemental data Public 41419_2019_1905_MOESM3_ESM.tif 126720 image/tiff 8c612b13-b38b-4794-ad54-8aecca7068d9 Supplemental data Public 41419_2019_1905_MOESM4_ESM.pdf 247798 application/pdf 0cd12ebc-0298-4537-9cc1-1d3c2330af5d Supplemental data Public 41419_2019_1905_MOESM5_ESM.pdf 327817 application/pdf 6a4e4bee-94e2-4813-ad22-dbdf1fa8e495 Supplemental data Public 41419_2019_1905_MOESM6_ESM.pdf 243845 application/pdf openAccess Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

Title	Published in	Access level	OA Policy	Year	Views	Downloads

The fine-tuning of endoplasmic reticulum stress response and autophagy activation during trophoblast syncytialization	Cell Death & Disease			2019	449	252

Archive ouverte UNIGE

Morris, Nolwenn