Ultrafast pulse shaping modulates perceived visual brightness in living animals

Article Article scientifique Article Ultrafast pulse shaping modulates perceived visual brightness in living animals eng 2021 author Gaulier Geoffrey 749322 author Dietschi Quentin 632833 author Bhattacharyya Swarnendu author Schmidt Cédric 678348 author Montagnese Matteo 884209 author Chauvet Adrien Alexis Paul 843064 author Hermelin Sylvain 793099 author Chiodini Florence 30216 author Bonacina Luigi 0000-0003-0476-4473 632447 author Herrera Pedro Luis 0000-0003-0771-9504 23981 author Rothlisberger Ursula author Rodriguez Ivan 28183 author Wolf Jean-Pierre 0000-0003-3729-958X 631266 eabe1911 10.1126/sciadv.abe1911 33910906 2375-2548 https://advances.sciencemag.org/lookup/doi/10.1126/sciadv.abe1911 Vision is usually assumed to be sensitive to the light intensity and spectrum but not to its spectral phase. However, experiments performed on retinal proteins in solution showed that the first step of vision consists in an ultrafast photoisomerization that can be coherently controlled by shaping the phase of femtosecond laser pulses, especially in the multiphoton interaction regime. The link between these experiments in solution and the biological process allowing vision was not demonstrated. Here, we measure the electric signals fired from the retina of living mice upon femtosecond multipulse and single-pulse light stimulation. Our results show that the electrophysiological signaling is sensitive to the manipulation of the light excitation on a femtosecond time scale. The mechanism relies on multiple interactions with the light pulses close to the conical intersection, like pump-dump (photoisomerization interruption) and pump-repump (reverse isomerization) processes. This interpretation is supported both experimentally and by dynamics simulations. Dewey 500.2 Dewey 576.5 Dewey 590 Swiss National Science Foundation Quantum Vision CRSII5_170981 CH Programmes 27 Section de physique 13 44 Département de génétique et évolution 162 135 Département de médecine génétique et développement 21D false 676 Groupe Wolf 272 Types cellulaires pancréatiques pendant l'ontogénèse 522 Science Advances 7 18 f828e25f-ce59-48da-b234-abad9298338f Article (Published version) Public Gaulier2021-1.pdf 481462 application/pdf CC BY-NC 58b36cc9-3f2c-4b88-9612-380f2fa6b55d Supplemental data Public abe1911_SM.pdf 790745 application/pdf CC BY-NC openAccess Faculté des sciences / Section de physique Faculté des sciences / Section de biologie / Département de génétique et évolution Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Ultrafast pulse shaping modulates perceived visual brightness in living animals	Science Advances			2021	460	246

Archive ouverte UNIGE

Montagnese, Matteo