Induction of a chromatin boundary in vivo upon insertion of a TAD border

Article Article scientifique Article Induction of a chromatin boundary in vivo upon insertion of a TAD border eng 2021-07-22 2021-07-22 author Willemin Andréa 0000-0002-4370-6914 832782 author Lopez-Delisle Lucille 0000-0002-1964-4960 author Bolt Christopher Chase 0000-0002-3544-3552 842881 author Gadolini Marie-Laure 883908 author Duboule Denis 0000-0001-9961-2960 28643 author Rodriguez Carballo Edgardo 0000-0003-4321-508X 819920 Département de biologie moléculaire et cellulaire (MOCEL) e1009691 10.1371/journal.pgen.1009691 34292939 1553-7390 PMC8330945 https://dx.plos.org/10.1371/journal.pgen.1009691 <p> Mammalian genomes are partitioned into sub-megabase to megabase-sized units of preferential interactions called topologically associating domains or TADs, which are likely important for the proper implementation of gene regulatory processes. These domains provide structural scaffolds for distant cis regulatory elements to interact with their target genes within the three-dimensional nuclear space and architectural proteins such as CTCF as well as the cohesin complex participate in the formation of the boundaries between them. However, the importance of the genomic context in providing a given DNA sequence the capacity to act as a boundary element remains to be fully investigated. To address this question, we randomly relocated a topological boundary functionally associated with the mouse HoxD gene cluster and show that it can indeed act similarly outside its initial genomic context. In particular, the relocated DNA segment recruited the required architectural proteins and induced a significant depletion of contacts between genomic regions located across the integration site. The host chromatin landscape was re-organized, with the splitting of the TAD wherein the boundary had integrated. These results provide evidence that topological boundaries can function independently of their site of origin, under physiological conditions during mouse development. </p> Dewey 590 European Research Council 588029 NICHD NIH HHS F32 HD093555 Eunice Kennedy Shriver National Institute of Child Health and Human Development F32HD093555 Swiss National Science Foundation Hox genes and the molecular bases of vertebrate development 138662 CH Projects European Commission A System Approach to Hox Genes Regulation in Vertebrates 232790 SYSTEMSHOX.CH EU FP7 44 Département de génétique et évolution 162 false PLOS genetics 17 7 ebf17afb-cee6-4700-8b30-9c62577e8392 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 2770091 application/pdf CC BY-4.0 8f33e22317d5429a5979a8dd29e7edb26340dc9386f67bbaa5c96b515966d66e openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Induction of a chromatin boundary in vivo upon insertion of a TAD border	PLOS genetics			2021	59	28

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Gadolini, Marie-Laure