Genomic study of endangered plant populations in the canton of Geneva

Diplôme Master Genomic study of endangered plant populations in the canton of Geneva eng 2023-08 author Cornide Nathan 880880 director Perret Mathieu 0000-0003-2021-114X 120893 Département des sciences végétales (BIVEG) director Naciri Yamama 0000-0001-6784-8565 503270 Département des sciences végétales (BIVEG) director Christe Camille 631727 director Price Michelle 556219 Département des sciences végétales (BIVEG) The genetic diversity of endangered plants is still largely unknown and rarely influences conservation plans. A perfect example of this situation is found in Geneva. Out of 270 threatened taxa, only two have been genetically studied. To provide new information on this essential genetic diversity, we used HTS approaches and the Hyb-Seq technique with the universal sequence capture probe set Angiosperms353. Being originally designed for phylogenetic studies, the potential of this probe set for conservation genomics, particularly for small, threatened populations, has been so far little explored. In this context, we analysed the population genetic diversity of three endangered species in Geneva: Balckstonia acuminata, Eryngium campestre and Lythrum hyssopifolia. On average, more than 900 unlinked SNPs were recovered. This number made it possible to analyse the genetic diversity, structure, and historical demography of the populations. Fst, although low, revealed significant differentiation between subpopulations. Notable heterozygosity for B. acuminata(0.95) and L. hyssopifolia (050) highlighted the possibility of hybridisation and polyploidy, respectively. In addition, Tajima's D detected bottleneck events for B. acuminata and E. campestre. PCA revealed significant distinctions between the two studied subpopulations of B. acuminata. Additionally, for E. campestre, the subpopulation located on the left side of the Rhône River exhibited a distinct clustering pattern compared to the subpopulations on the right side of the Rhône. By contrast, only E. campestre showed clustering in the STRUCTURE analyses. Discussions on the most appropriate conservation strategies for these species are guided by these new insights. Dewey 580 45 Département des sciences végétales 163 false f51dcf74-8b4c-4c24-aadd-84d7684b9168 Master thesis Restricted CORNIDE_master_thesis_FINAL.pdf 30773852 application/pdf 5b78796a709e56a98f944c89d6e20a9f48503de56ff10a827be7c7282c41d95b restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Genomic study of endangered plant populations in the canton of Geneva				2023	86	3

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Cornide, Nathan

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