ERα Responses in Different Cellular States of Breast Cancer

Diplôme Master ERα Responses in Different Cellular States of Breast Cancer eng 2019 author Apostolopoulou Aikaterini 880650 director Picard Didier 0000-0001-8816-9668 26830 ERα is a central transcription factor, activated by ligand-dependent (estradiol [E2]) or –independent pathways (e.g. cAMP). ERα is expressed in two thirds of all breast tumors (ERα-positive). The final stage of breast cancer is metastasis. In order to better study breast cancer and its progression, the use of three-dimensional (3D) cultures combines both the utility of in vitro models with the advantages of systems closer to physiological conditions and the tumor microenvironment. During this study, ERα responses in different cellular states were evaluated in the ERα+ breast cancer cell lines MDA-MB-134 and MCF-7. The relationship between ERα and an EMT-inducing transcription factor (EMT-TF), ZEB1, was studied when ERα is activated with both ways and between classical 2D and 3D cell cultures. Exogenous ZEB1 expression in ERα+ breast cancer cells increases cell migration and invasion. 2D and 3D cell culture methods delivered significantly different results in comparison. Estrogen Receptor (ER) Estrogen Three-dimensional (3D) cell culture EMT CAMP Dewey 570 46 Département de biologie cellulaire 165 false f3491036-2867-47a0-838c-ec802370fec5 Master thesis Restricted Apostolopoulou.Katerina_Master.Thesis_17.09.19.pdf 3181794 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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ERα Responses in Different Cellular States of Breast Cancer				2019	90	2

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Apostolopoulou, Aikaterini