Facile Synthesis, Size-Separation, Characterization, and Antimicrobial Properties of Thiolated Copper Clusters

Article Article scientifique Article Facile Synthesis, Size-Separation, Characterization, and Antimicrobial Properties of Thiolated Copper Clusters eng 2018 author Baghdasaryan Ani 804897 author Grillo Rossella 842596 author Bhattacharya Sarita 875178 author Sharma Manish 795073 author Reginato Emilie author Theraulaz Harry 765229 author Dolamic Igor 749175 author Dadras Massoud author Rudaz Serge 0000-0002-4180-5417 28978 author Varesio Emmanuel 30230 author Buergi Thomas 727741 4258-4267 10.1021/acsanm.8b01049 2574-0970 https://pubs.acs.org/doi/10.1021/acsanm.8b01049 Metal nanomaterials have attracted extensive attention in biological labeling and imaging due to their controllable physical and chemical properties. Recently, a lot of effort has been devoted to preparing various ultrasmall and functional copper nanoclusters (CuNCs) with different emissions from blue to red, soluble both in organic and aqueous phases. Herein, a novel one-step synthetic method is proposed for the preparation of stable water-soluble glutathione-capped (GSH-capped) CuNCs. The resulting nanoclusters have a good dispersibility and stability in aqueous media. The stability was examined by several test experiments. The mass detection in ESI-HRMS mode allowed ionization of several doubly charged species with formula Cu5L6, Cu6L6, Cu7L6, Cu8L6, and Cu9L6 (L = C10H16N3O6S). The use of advanced separation techniques including liquid chromatography (HPLC), gel electrophoresis (PAGE), and capillary electrophoresis (CE) allowed the separation of several clusters, some of which are larger than Au25(GS)18, as shown by PAGE. To the best of our knowledge, this is the first report on successful size-separation of CuNCs. Moreover, the synthesized CuNCs show a dose-dependent antimicrobial effect. At lower cluster concentration the growth of bacteria is partially reduced. However, at higher concentrations, the bacterial growth is completely restricted. Copper nanoclusters ESI-HRMS HPLC PAGE Capillary electrophoresis (CE) Dewey 540 Dewey 615 38 Département de chimie physique 142 31 Section des sciences pharmaceutiques 17 false ACS Applied Nano Materials 1 8 0858eeef-7b52-45a9-90d0-0349e600f217 Article (Published version) Restricted Facile_TB_18.pdf 3969363 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie physique Faculté des sciences / Section des sciences pharmaceutiques

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Facile Synthesis, Size-Separation, Characterization, and Antimicrobial Properties of Thiolated Copper Clusters	ACS Applied Nano Materials	2018	294	1

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Bhattacharya, Sarita