Induction of Chalcone Synthase Expression by Rhizobia and Nod factors in Root Hairs and Roots

Article Article scientifique Article Induction of Chalcone Synthase Expression by Rhizobia and Nod factors in Root Hairs and Roots eng 1997 author Krause Andréa 24341 author Vo Thi Thuong Lan 87189 author Broughton William John 22724 388-393 10.1094/MPMI.1997.10.3.388 0894-0282 http://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.3.388 Chalcone synthase (CHS) of Vigna unguiculata is encoded by a gene family that is abundantly transcribed in leaves and nodules. Inoculation with Rhizobium sp. NGR234, which nodulates V. unguiculata, or with NGRΔnodABC, a mutant deficient in Nod factor production, induced rapid accumulation of CHS mRNAs in roots and root hairs. As both Nod⁺ and Nod⁻ bacteria provoke responses, induction of CHS gene expression may involve symbiotic or defense responses. Four days after inoculation with the wild-type Rhizobium sp., the transcript levels increased in roots but decreased in root hairs. Use of a region unique to the 5′ end of a specific CHS gene (VuCHS1) showed that increases of transcript levels in root hairs 24 h after inoculation with both rhizobia were specific to this gene. Transcripts of this gene in roots were only detectable 4 days after treatment with NGR234. It is possible therefore that accumulation of VuCHS1 follows the infection pathway of rhizobia entering legume roots. Purified Nod factors induced accumulation of transcripts, showing that they might be part of the signal transduction pathway leading to CHS expression. Symbiosis Dewey 580 45 Département des sciences végétales 163 false Molecular Plant-Microbe Interactions 10 3 d7caf5c6-350c-4b10-9b93-afeb02344751 Article (Published version) Public mpmi.1997.10.3.388-1.pdf 240657 application/pdf OA OTHER openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Induction of Chalcone Synthase Expression by Rhizobia and Nod factors in Root Hairs and Roots	Molecular Plant-Microbe Interactions			1997	346	399

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Vo, Thi Thuong Lan