Mechanisms and Persistence of Chromatin-Mediated Antisense Transcription Interference

Diplôme Thèse Mechanisms and Persistence of Chromatin-Mediated Antisense Transcription Interference eng 2020-10-16 author Kaur Jatinder 866224 director Stutz Françoise 64881 director Soudet Julien 824053 10.13097/archive-ouverte/unige:148174 urn:nbn:ch:unige-1481743 Sc. Vie 80 Eukaryotic genomes are transcribed almost entirely in an interleaved manner giving rise to manynon-coding RNA (ncRNA) transcripts overlapping coding gene promoters. In the compact genome ofthe yeast S.cerevisiae, these non-coding transcripts end up being in antisense orientation to theupstream mRNA. Nuclear surveillance mechanisms restrict non-coding transcription through early-termination and degradation by Nrd1/Nab3/Sen1(NNS) pathway. The act of non-coding RNAtranscription by itself causes gene repression by a poorly understood mechanism of transcriptioninterference (TI). The thesis discusses a chromatin-mediated mechanism of transcriptioninterference. It begins with Antisense-mediated co-transcriptional deposition of H3K36 methylationat sense promoter and Rpd3 histone deacetylase recognizes and deacetylates the promoter. This leads to closing of the promoter NDR due to reduced occupancy of RSC chromatin remodeler, that restrictsTBP binding and results in TI. Furthermore, persistence of antisense-mediated repression is also addressed. Non-coding transcription Transcription interference Promoter Nucleosome-Depleted Region Histone deacetylase Histone methyltransferase Nucleosome positioning Dewey 570 iGE3 grant 46 Département de biologie cellulaire 165 false 839 Groupe Stutz 6a32ace6-87c8-4200-a5e6-e6369e90bfc7 Thesis Public Thesis_Kaur_Jatinder.pdf 29400017 application/pdf Restricted 2022-01-11 bc703983adbf41f6596261bfe7bcd911dfc37e9788b15d28b9063f895f635a1b openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Mechanisms and Persistence of Chromatin-Mediated Antisense Transcription Interference				2020	522	279

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Kaur, Jatinder