Studying the Mitochondrial RNA granules: structure, compartmentalisation and regulation of their molecular content

Diplôme Thèse Studying the Mitochondrial RNA granules: structure, compartmentalisation and regulation of their molecular content eng 2021-11-24 2021-11-29 author Xavier Vanessa Joanne 866223 director Martinou Jean-Claude 27293 151 10.13097/archive-ouverte/unige:158187 urn:nbn:ch:unige-1581877 Sc. Vie 130 We decided to study the interactions between the Mitochondrial RNA granules (MRGs) and the mt-dsRNA foci using super-resolution STED microscopy. We found that dsRNA foci are part of the MRGs, where they form a distinct, but overlapping nanodomain with the ss-RNA core of MRGs. Furthermore, dsRNA foci colocalize with GRSF1 and to a lesser extent with FASTKD2, two protein components of MRGs. In addition, we show that the degradosome component SUV3 has a dual localization, within and outside MRGs.It had also been reported that mt-dsRNA is undetectable in primary cells unlike in cancer cells. To investigate this observation further, we generated an in vitro model of cancer where human primary dermal fibroblasts have been transformed into malignant cells. We determined that dsRNA foci were observed after the induction of oncogenes. The process of tumourigenesis increased the total amount of MRGs, mtDNA and mitochondrial mass and mt-transcription. We posited that the total cellular proliferation of carcinogenic cells was linked to the increase in transcription and regulation of the mitochondrial genome. Dewey 570 46 Département de biologie cellulaire 165 false 837 Groupe Martinou 278b05b2-45ea-44a2-9578-9b6205096810 Thesis Restricted Thesis_XAVIER Vanessa_2022.pdf 7894351 application/pdf Private 2022-12-31 866c5a6587ffc85e50515347d16c73777ddbc6dc97b98a2a7552b73cee72495b restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Xavier, Vanessa Joanne

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