Fluorescence-activated cell sorting to reveal the cell origin of radioligand binding

Article Article scientifique Article Fluorescence-activated cell sorting to reveal the cell origin of radioligand binding eng 2019 author Tournier Benjamin 705309 author Tsartsalis Stergios 0000-0002-6565-6313 782369 author Ceyzeriat Kelly 910236 author Medina Callahui Zadith Evelyn 865709 author Fraser Ben H author Grégoire Marie-Claude author Kovari Eniko Veronika 501395 author Millet Philippe 0000-0002-5803-0478 668343 0271678X1986040 10.1177/0271678X19860408 31242048 0271-678X Many studies have explored the role of TSPO (18 kDa translocator protein) as a marker of neuroinflammation using single-photon emission computed tomography (SPECT) or positron emission tomography (PET). In vivo imaging does not allow to determine the cells in which TSPO is altered. We propose a methodology based on fluorescence-activated cell sorting to sort different cell types of radioligand-treated tissues. We compared left/right hippocampus of rats in response to a unilateral injection of lipopolysaccharide (LPS), ciliary neurotrophic factor (CNTF) or saline. We finally applied this methodology in human samples (Alzheimer's disease patients and controls). Our data show that the pattern of TSPO overexpression differs across animal models of acute neuroinflammation. LPS induces a microglial expansion and an increase in microglial TSPO binding. CNTF is associated with an increase in TSPO binding in microglia and astrocytes in association with an increase in the number of microglial binding sites per cell. In humans, we show that the increase in CLINDE binding in Alzheimer's disease concerns microglia and astrocytes in the presence of a microglial expansion. Thus, the cellular basis of TSPO overexpression is condition dependent, and alterations in TSPO binding found in PET/SPECT imaging studies cannot be attributed to particular cell types indiscriminately. Alzheimer's disease Fluorescence-activated cell sorting Neuroinflammation Positron emission tomography/single-photon Emission computed tomography Translocator protein Dewey 616.0757 Dewey 616.89 Swiss National Science Foundation Molecular Imaging of Neuroinflammation in Bipolar Disorder: a Translational Validation of the TSPO Quantification Methodology 184713 CH Project funding 60 Département de psychiatrie 225 62 Département de radiologie et informatique médicale 227 false 1221 Neuroimagerie moléculaire en psychiatrie 983 Journal of Cerebral Blood Flow and Metabolism 68a2fbe6-ea60-45ec-8d89-77883ac49747 Article (Published version) Restricted 0271678x19860408.pdf 1733189 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de psychiatrie Faculté de médecine / Section de médecine clinique / Département de radiologie et informatique médicale

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Fluorescence-activated cell sorting to reveal the cell origin of radioligand binding	Journal of Cerebral Blood Flow and Metabolism			2019	542	1

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Medina Callahui, Zadith Evelyn