Deterministic actin waves as generators of cell polarization cues

Article Article scientifique Article Deterministic actin waves as generators of cell polarization cues eng 2020 author Stankevicins Luiza author Ecker Nicolas 865610 author Terriac Emmanuel author Maiuri Paolo author Schoppmeyer Rouven author Vargas Pablo author Lennon-Duménil Ana-Maria author Piel Matthieu author Qu Bing author Hoth Markus author Kruse Karsten 861969 author Lautenschläger Franziska 826-835 10.1073/pnas.1907845117 0027-8424 http://www.pnas.org/lookup/doi/10.1073/pnas.1907845117 Dendritic cells “patrol” the human body to detect pathogens. In their search, dendritic cells perform a random walk by amoeboid migration. The efficiency of pathogen detection depends on the properties of the random walk. It is not known how the dendritic cells control these properties. Here, we quantify dendritic cell migration under well-defined 2-dimensional confinement and in a 3-dimensional collagen matrix through recording their long-term trajectories. We find 2 different migration states: persistent migration, during which the dendritic cells move along curved paths, and diffusive migration, which is characterized by successive sharp turns. These states exhibit differences in the actin distributions. Our theoretical and experimental analyses indicate that this kind of motion can be generated by spontaneous actin polymerization waves that contribute to dendritic cell polarization and migration. The relative distributions of persistent and diffusive migration can be changed by modification of the molecular actin filament nucleation and assembly rates. Thus, dendritic cells can control their migration patterns and adapt to specific environments. Our study offers an additional perspective on how dendritic cells tune their searches for pathogens. Dewey 500.2 Dewey 540 37 Département de physique théorique 133 39 Département de biochimie 144 false Proceedings of the National Academy of Sciences 117 2 fd16201c-8ecc-4006-b5c6-2f7279e7c1f5 Article (Published version) Restricted Deterministic_KK_20.pdf 2046183 application/pdf restrictedAccess Faculté des sciences / Section de physique / Département de physique théorique Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Excitable actin dynamics and amoeboid cell migration	PLOS ONE	2021	397	224
Deterministic actin waves as generators of cell polarization cues	Proceedings of the National Academy of Sciences	2020	415	0
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Ecker, Nicolas