Quantification and Phenotypic Characterization of Extracellular Vesicles from Patients with Acute Myeloid and B-Cell Lymphoblastic Leukemia

Article Article scientifique Article Quantification and Phenotypic Characterization of Extracellular Vesicles from Patients with Acute Myeloid and B-Cell Lymphoblastic Leukemia eng 2021-12-23 2021-12-23 author Licina Marijana 0000-0001-9023-2208 133516 author Arraud Nicolas 865116 author Zahra Aicha Dorra 852035 author Ropraz Patricia 26300 author Matthes Thomas 27368 10.3390/cancers14010056 35008226 2072-6694 PMC8750511 Extracellular vesicles (EVs) act in cell-to-cell communication, delivering cargo from donor to recipient cells and modulating their physiological condition. EVs secreted by leukemic blasts in patients with leukemia have been shown to influence the fate of recipient cells in the bone marrow microenvironment. Methods to quantify and to characterize them phenotypically are therefore urgently needed to study their functional role in leukemia development and to evaluate their potential as targets for therapy. We have used cryo-electron microscopy to study morphology and size of leukemic EVs, and nanoparticle tracking analysis and fluorescence triggering flow cytometry to quantify EVs in platelet-free plasma from a small cohort of leukemia patients and healthy blood donors. Additional studies with a capture bead-based assay allowed us to establish phenotypic signatures of leukemic EVs from 17 AML and 3 B-ALL patients by evaluating the expression of 37 surface antigens. In addition to tetraspanins and lineage-specific markers we found several adhesion molecules (CD29, and CD146) to be highly expressed by EVs from B-ALL and several leukemic stem cell antigens (CD44, CD105, CD133, and SSEA-4) to be expressed by EVs from AML patients. Further improvements in analytical methods to study EVs are needed before potentially using them as biomarkers for leukemia prognosis and follow-up. AML B-ALL Acute leukemia Extracellular vesicles Flow cytometry Dewey 616 CSRD VA 1 United States Dr. Henri Dubois-Ferrière Dinu Lipatti 2 56 Département de médecine 221 false 586 Analyses cellulaires et moléculaires des hémopathies malignes 929 Cancers 14 1 fd580b4f-de98-49c3-9581-5ea3fab5f99f Article (Published version) Public Article-scientifique-from-unpaywall.pdf 3249316 application/pdf CC BY c578cecf-d525-407a-b9c6-1366012ccc6b Appendix Public cancers-14-00056-s001(2).zip 872498 application/zip openAccess Faculté de médecine / Section de médecine clinique / Département de médecine

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Quantification and Phenotypic Characterization of Extracellular Vesicles from Patients with Acute Myeloid and B-Cell Lymphoblastic Leukemia	Cancers			2021	218	100

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Arraud, Nicolas