Assessment of tools to improve microscopy for the detection of Plasmodium infections in research contexts

Diplôme Thèse Assessment of tools to improve microscopy for the detection of Plasmodium infections in research contexts eng 2022-06-15 author Das Debashish 862901 director Chappuis François 34709 director Guerin Philippe J. 10.13097/archive-ouverte/unige:166216 urn:nbn:ch:unige-1662161 Sc. Bioméd. - S. Glob. 021 <p>Microscopy is essential for malaria diagnosis, parasite species differentiation, and density estimation. A systematic literature review was performed to explore how microscopy methods were reported in published malaria studies (2013-2017). Consequently, an evidence-based checklist for reporting microscopy procedures has been proposed.</p> <p>Malaria microscopy is undermined by difficulties in ensuring high-quality manual reading and inter-reader reliability. A multi-centre, observational study was conducted in 2018-19 to assess the diagnostic performance of EasyScan Go, a machine-learning-based microscope. Of 2,250 blood films evaluated, the diagnostic sensitivity of EasyScan Go was 91.1% (95%CI 88.9-92.7), and specificity 75.6% (95%CI 73.1-78.0). Species were identified accurately in 93% of P. falciparum (kappa=0.76, 95%CI 0.69-0.83), and in 92% of P. vivax samples (kappa=0.73, 95%CI 0.66-0.80). Parasite density estimates by EasyScan Go were within +/- 25% of the microscopic reference counts in 23% of slides. Further software improvement is required to improve sensitivity at low parasitaemia and parasite density estimations.</p> Dewey 613 66 Institut de santé globale 22A1 false a7fb53a4-5720-44a0-93e4-432a8fd93019 Thesis Public Thesis Dissertation_Debashish Das_1.pdf 1694594 application/pdf af8b9b69-f921-4946-890a-e59cb7409b9d Appendix Public Additional File.pdf 302442 application/pdf e5e14cc0-bd78-4011-88ac-307be3f49b94 Appendix Public Article 1.pdf 331933 application/pdf 1437bd79-55da-43b6-8ea0-601a3265f0d2 Appendix Public Article 2.pdf 1889504 application/pdf 98c0983c-3f8e-4ab1-83b1-d3ae5944745c Imprimatur Public Signed_Imprimatur_DD.pdf 281288 application/pdf openAccess Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de santé globale

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Das, Debashish