Functional and anatomical characterization of atrophic age-related macular degeneration in an aged mouse model

Article Article scientifique Article Functional and anatomical characterization of atrophic age-related macular degeneration in an aged mouse model eng 2021 author Tobalem Stéphan Jonathan 894433 author Kecik Mateusz Mariusz 849583 author Midroit Maëllie 893525 author Scaldino Djehna 861566 author Tardy Sébastien 784523 author Bascuas Castillo Thaïs 0000-0002-3011-2201 893012 author Harmening Nina 794769 author Scapozza Leonardo 600318 author Thumann Gabriele 0000-0003-3682-9569 785873 author Kropp Martina 0000-0001-6861-5851 793095 128-152 10.26502/fjor.2644-00240032 2644-0024 https://www.fortunejournals.com/abstract/functional-and-anatomical-characterization-of-atrophic-agerelated-macular-degeneration-in-an-aged-mouse-model-2187.html Purpose: To develop an animal model of atrophic age-related macular degeneration (aAMD) in aged mice that more closely mimics the natural progression of the disease. Methods: 12- and 18-month-old CBl57/6JRj mice were immunized with murine serum albumin (MSA) conjugated with carboxyethyl pyrrole (CEP). The immunization, given into the hock, was followed by 3 booster injections into the neck over a 3-month period. Immunized mice and age-matched controls were trained for a visual discrimination and an optokinetic response task to determine the objective visual threshold (OVT) at arrival and at 3 months; funduscopy and ocular coherence tomography were performed. After sacrifice, the eyes were enucleated for histological, immunofluorescent and electron microscopy analyses. Results: Retinal imaging confirmed that all mice had normal retinas upon arrival. Three months after mice were immunized the normal retinal age-related alterations were significantly more pronounced in CEP-immunized than in control mice as evidenced by drusenoid alterations, increased retinal thickness, immune activation, signs of retinal degeneration, decreased OVT. Electron microscopy indicated degeneration of the retinal pigment epithelium (RPE). Conclusions: The retinal and behavioral changes in the aged CEP-immunized mice will be useful for the investigation of novel treatments of aAMD. Translational relevance: The enhanced Aged-CEP-Mouse model enables the generation of results highly transferable to human patients and promotes the development of efficient, safe AMD therapies. Atrophic Age-Related Macular Degeneration Mouse model Dewey 615 Dewey 616.8 Swiss National Science Foundation Transposon Based Gene Cell Therapy In The Treatment Of Dry Macular Degeneration 31003A_160195 CH Project funding 31 Section des sciences pharmaceutiques 17 61 Département des neurosciences cliniques 226 false 880 Biochimie pharmaceutique 582 Ophtalmologie expérimentale 925 Journal of Ophthalmology and Research 4 2 f78d1ecb-0c23-4914-a833-ca78510434e6 Article (Published version) Public Tobalem_functional-and-anatomical_2021.pdf 1784921 application/pdf CC BY openAccess Faculté des sciences / Section des sciences pharmaceutiques Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques

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Functional and anatomical characterization of atrophic age-related macular degeneration in an aged mouse model	Journal of Ophthalmology and Research			2021	465	391

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Scaldino, Djehna