The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis

Article Article scientifique Article The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis eng 2018 author Chahtane Hicham 830334 author Fuller Thanise 858533 author Allard Pierre-Marie 812151 author Marcourt Laurence 729234 author Ferreira Queiroz Emerson 135675 author Shanmugabalaji Venkatasalam author Falquet Jacques 22472 author Wolfender Jean-Luc 135649 author Lopez Molina Luis 0000-0003-0463-1187 28870 10.7554/eLife.37082 30149837 2050-084X To anticipate potential seedling damage, plants block seed germination under unfavorable conditions. Previous studies investigated how seed germination is controlled in response to abiotic stresses through gibberellic and abscisic acid signaling. However, little is known about whether seeds respond to rhizosphere bacterial pathogens. We found that Arabidopsis seed germination is blocked in the vicinity of the plant pathogen Pseudomonas aeruginosa. We identified L-2-amino-4-methoxy-trans-3-butenoic acid (AMB), released by P. aeruginosa, as a biotic compound triggering germination arrest. We provide genetic evidence that in AMB-treated seeds DELLA factors promote the accumulation of the germination repressor ABI5 in a GA-independent manner. AMB production is controlled by the quorum sensing system IQS. In vitro experiments show that the AMB-dependent germination arrest protects seedlings from damage induced by AMB. We discuss the possibility that this could serve as a protective response to avoid severe seedling damage induced by AMB and exposure to a pathogen. Abscisic Acid/metabolism Aminobutyrates/pharmacology Antibody Specificity Arabidopsis/drug effects/embryology/genetics/microbiology Arabidopsis Proteins/metabolism Discriminant Analysis Gene Expression Regulation Plant/drug effects Germination/drug effects Gibberellins/pharmacology Glycine/analogs & derivatives/pharmacology Least-Squares Analysis Metabolomics Pseudomonas aeruginosa/physiology Seeds/drug effects/embryology Signal Transduction/drug effects Triazoles/pharmacology Dewey 580 Dewey 615 45 Département des sciences végétales 163 31 Section des sciences pharmaceutiques 17 false 831 Groupe Lopez-Molina 882 Phytochimie et produits naturels bioactifs eLife 7 10ebdd5c-e871-4748-aa2a-12f6f81417da Article (Published version) Public Chahtane_et_al_2018.pdf 5132614 application/pdf openAccess Faculté des sciences / Section de biologie / Département des sciences végétales Faculté des sciences / Section des sciences pharmaceutiques

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The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis	eLife			2018	423	316

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