Identification of a micropeptide and multiple secondary cell genes that modulate drosophila male reproductive success

Article Article scientifique Article Identification of a micropeptide and multiple secondary cell genes that modulate drosophila male reproductive success eng 2021-04-05 2021-04-05 author Immarigeon Clément 0000-0001-7985-5885 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 840261 author Frei Yohan 0000-0002-4600-5072 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 705098 Yohan El Bali author Delbare Sofie Y.N. 0000-0001-7964-3962 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703; author Gligorov Dragan 0000-0002-3153-5003 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 651985 author Machado Almeida Pedro 0000-0003-4423-7707 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 675906 author Grey Jasmine 0000-0001-6095-4461 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703; author Fabbro Léa Kim 0000-0001-7575-1165 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 858213 author Nagoshi Emi 0000-0002-6365-4074 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 535940 Département de génétique et évolution (Genev) (BIANI) author Billeter Jean-Christophe 0000-0001-7796-4068 Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen 9700 CC, The Netherlands author Wolfner Mariana F. 0000-0003-2701-9505 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703; author Karch François 0000-0003-4722-5170 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 26338 Département de génétique et évolution (Genev) (BIANI) author Maeda Robert 0000-0001-6555-1215 Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; 539408 Département de génétique et évolution (Genev) (BIANI) 1-11 e2001897118 10.1073/pnas.2001897118 33876742 0027-8424 PMC8053986 https://pnas.org/doi/full/10.1073/pnas.2001897118 Even in well-characterized genomes, many transcripts are considered noncoding RNAs (ncRNAs) simply due to the absence of large open reading frames (ORFs). However, it is now becoming clear that many small ORFs (smORFs) produce peptides with important biological functions. In the process of characterizing the ribosomebound transcriptome of an important cell type of the seminal fluid-producing accessory gland of Drosophila melanogaster, we detected an RNA, previously thought to be noncoding, called male-specific abdominal (msa). Notably, msa is nested in the HOX gene cluster of the Bithorax complex and is known to contain a micro-RNA within one of its introns. We find that this RNA encodes a “micropeptide” (9 or 20 amino acids, MSAmiP) that is expressed exclusively in the secondary cells of the male accessory gland, where it seems to accumulate in nuclei. Importantly, loss of function of this micropeptide causes defects in sperm competition. In addition to bringing insights into the biology of a rare cell type, this work underlines the importance of small peptides, a class of molecules that is now emerging as important actors in complex biological processes. Drosophila Accessory gland Postmating response Reproduction SmORF peptide Dewey 590 NICHD NIH HHS R01 HD059060 44 Département de génétique et évolution 162 false Proceedings of the National Academy of Sciences of the United States of America 118 15 06fc7cb6-4b84-4fb2-b9a1-81b23e6501db Article (Published version) Public Article-scientifique-from-unpaywall.pdf 2053943 application/pdf CC BY-NC-ND-4.0 db33773ee9a359e5355624c46f40db5f6848db8295af16762b275e7d3ef235fa openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Identification of a micropeptide and multiple secondary cell genes that modulate drosophila male reproductive success	Proceedings of the National Academy of Sciences of the United States of America			2021	98	39

Archive ouverte UNIGE

Fabbro, Léa Kim