Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain‐containing proteins EXD1 and EXD2 in mouse spermatocytes

Article Article scientifique Article Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain‐containing proteins EXD1 and EXD2 in mouse spermatocytes eng 2023-01-09 2023-01-09 author Olotu Opeyemi Institute of Biomedicine, Integrative Physiology and Pharmacology Unit University of Turku Turku Finland author Dowling Mark 0009-0006-2091-0724 Department of Molecular Biology, Science III University of Geneva Geneva Switzerland 877696 Département de biologie moléculaire et cellulaire (MOCEL) author Homolka David Department of Molecular Biology, Science III University of Geneva Geneva Switzerland 856314 Département de biologie moléculaire et cellulaire (MOCEL) author Wojtas Magdalena Natalia Department of Molecular Biology, Science III University of Geneva Geneva Switzerland 858019 author Tran Panyi Institute of Biomedicine, Integrative Physiology and Pharmacology Unit University of Turku Turku Finland author Lehtiniemi Tiina Institute of Biomedicine, Integrative Physiology and Pharmacology Unit University of Turku Turku Finland author Da Ros Matteo Institute of Biomedicine, Integrative Physiology and Pharmacology Unit University of Turku Turku Finland author Pillai Ramesh Department of Molecular Biology, Science III University of Geneva Geneva Switzerland 854560 Département de biologie moléculaire et cellulaire (MOCEL) author Kotaja Noora 0000-0003-1503-9170 Institute of Biomedicine, Integrative Physiology and Pharmacology Unit University of Turku Turku Finland 710-723 10.1111/andr.13361 36624638 2047-2919 https://onlinelibrary.wiley.com/doi/10.1111/andr.13361 Background Germ granules are large cytoplasmic ribonucleoprotein complexes that emerge in the germline to participate in RNA regulation. The two most prominent germ granules are the intermitochondrial cement (IMC) in meiotic spermatocytes and the chromatoid body (CB) in haploid round spermatids, both functionally linked to the PIWI‐interacting RNA (piRNA) pathway. Aims In this study, we clarified the IMC function by identifying proteins that form complexes with a well‐known IMC protein PIWIL2/MILI in the mouse testis. Results The PIWIL2 interactome included several proteins with known functions in piRNA biogenesis. We further characterized the expression and localization of two of the identified proteins, Exonuclease 3′–5′ domain‐containing proteins EXD1 and EXD2, and confirmed their localization to the IMC. We showed that EXD2 interacts with PIWIL2, and that the mutation of Exd2 exonuclease domain in mice induces misregulation of piRNA levels originating from specific pachytene piRNA clusters, but does not disrupt male fertility. Conclusion Altogether, this study highlights the central role of the IMC as a platform for piRNA biogenesis, and suggests that EXD1 and EXD2 function in the IMC‐mediated RNA regulation in postnatal male germ cells. EXD1 EXD2 Chromatoid body Intermitochondrial cement PiRNA, spermatogenesis Dewey 570 43 Département de biologie moléculaire 161 false Andrology 11 4 3c8d7911-8cf0-4529-84c4-29cdd8b6d546 Article (Published version) Public Andrology - 2023 - Olotu - Intermit.pdf 6157222 application/pdf CC BY-4.0 10e981d4e95a151a1259069236ee51ebf2138c8cfa5a8f3150c253914af74ef1 openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

Title	Published in	Year	Views	Downloads

Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain‐containing proteins EXD1 and EXD2 in mouse spermatocytes	Andrology	2023	70	43
Regulation of m⁶A Transcripts by the 3ʹ→5ʹ RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline	Molecular cell	2017	73	201

Archive ouverte UNIGE

Wojtas, Magdalena Natalia