Study of Supporting-like cells in adult mouse testis by DTR-induced ablation of Pax8-expressing cells

Diplôme Master Study of Supporting-like cells in adult mouse testis by DTR-induced ablation of Pax8-expressing cells eng 2024-09-25 author Cottier Syrielle Yashvina 857986 director Nef Serge 0000-0001-5462-0676 29062 Département de médecine génétique et développement (GEDEV) Recent single-cell RNA sequencing of mouse XX and XY gonads across gonadal sex determination identified a rare cell population, designated as supporting-like cells (SLCs). SLCs primarily constitute the epithelium of the rete testis and ovarii in XY and XX gonads, respectively. The rete testis serves as an anatomical bridge between the testis and the efferent ducts, facilitating spermatozoa transport to the epididymis. The rete testis is composed of two distinct portions: an intratesticular and an extratesticular one. In the embryonic and neonatal testis, SLCs form the epithelium from the extratesticular rete testis to the straight tubules. SLCs represent the sole gonadal cell population expressing the transcription factor Pax8. The present study sought to elucidate the putative functions of rete testis epithelial cells in adult mice. This involved the utilisation of a genetic cell ablation technique based on differential sensitivity to diphtheria toxin, coupled with exploitation of the Pax8-specific expression in SLCs. It was found that only the intragonadal SLCs were able to undergo cell ablation efficiently. The ablation of intragonadal SLCs in mice results in efferent duct ligation-like effects, suggesting that these cells may be involved in the reabsorption of testis fluid. Dewey 590 Dewey 576.5 Maitrise universitaire (master) en biologie: Genetic, Development and Evolution (GDE) 44 Département de génétique et évolution 162 135 Département de médecine génétique et développement 21D false 448 Mécanismes moléculaires et génétiques du développement sexuel 778 7472af7b-7190-43a5-b9fe-6d2d325afa3c Master thesis Restricted Master_thesis_Syrielle_Yashvina_Cot.pdf 5204727 application/pdf 735065e4e661383b8a2148aa177b400eee063674383334fdf9dd5a3a5a69a8f2 restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

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Study of Supporting-like cells in adult mouse testis by DTR-induced ablation of Pax8-expressing cells				2024	64	4

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Cottier, Syrielle Yashvina

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