Optimization of the Hydrolysis of Freshwater Polysaccharides

Article Article scientifique Article Optimization of the Hydrolysis of Freshwater Polysaccharides eng 2000-08 author Wilkinson Kevin 29725 author Cuenoud François 85254 323-335 10.1080/03067310008032700 0306-7319 http://www.tandfonline.com/doi/abs/10.1080/03067310008032700 Three techniques of acid hydrolysis are verified for their ability to efficiently hydrolyse freshwater polysaccharides. Polysaccharides isolated from two eutrophic lakes were hydrolysed rapidly as compared to model or marine polysaccharides. No single technique is 100% satisfactory for all polysaccharides (i.e. complete hydrolysis with no degradation). Hydrolysis by 0.1 M HCl appeared to give the best results while acceptable results were also obtained for 1.2 M H2SO4. The results suggest that it is prudent to hydrolyse freshwater polysaccharides under the least drastic conditions and for as short of a time as possible in order to minimize decomposition of the sample, (esp. uronic acids). Interferences due to humic acid; did not appear to increase as a function of hydrolysis time. Polysaccharide Hydrolysis Dextran Xanthan Glucose Analytical determination Dewey 540 40 Département de chimie minérale, analytique et appliquée 145 false International journal of environmental analytical chemistry 77 4 74a7216b-95d6-46c1-965e-3661a548df1c Article (Published version) Restricted Wilkinson_IJEAC_200077323.pdf 4336259 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie minérale, analytique et appliquée

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Optimization of the Hydrolysis of Freshwater Polysaccharides	International journal of environmental analytical chemistry			2000	147	0

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Cuenoud, François