Hairy roots of Datura candida × D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis

Article Article scientifique Article Hairy roots of Datura candida × D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis eng 1998-03-09 author Nussbaumer Philippe 84779 author Kapetanidis Ilias 24938 author Christen Philippe 23100 405-409 10.1007/s002990050415 30736580 0721-7714 A transformed root clone of Datura candida × D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg’s B5 medium supplemented with 5% sucrose was the best for root growth (288 mg dry weight/flask) and full-strength B5 medium for hyoscyamine and scopolamine content (0.36 and 0.17% dry weight, respectively). Experiments with exogenous nitrate added to the medium revealed that the biomass increased (353 mg dry weight/flask) and the hyoscyamine content improved remarkably (0.54% dry weight), but that the scopolamine content was significantly reduced. The addition of various precursors at two different concentrations did not significantly modify root growth. Feeding (R,S)- phenyllactic acid stimulated the biosynthesis of both alkaloids, whereas the addition of ornithine specifically reduced the scopolamine content. Hyoscyamine Medium optimization Scopolamine Transformed roots Datura candida × D. aurea Dewey 615 31 Section des sciences pharmaceutiques 17 false Plant cell reports 17 5 93a30fc7-0aba-4970-ab7e-933867047395 Article (Published version) Public Nussbaumer_PCR_199817405.pdf 57060 application/pdf OA NATIONAL 07ccc565ee319b03ee7cdbae358e52d250aea9e57c26fad154f0b6ce20444177 openAccess Faculté des sciences / Section des sciences pharmaceutiques

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Hairy roots of Datura candida × D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis	Plant cell reports			1998	98	136

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Nussbaumer, Philippe