Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

Article Article scientifique Article Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection eng 2018 author Sayers Jessica 843420 author Payne Richard J. author Winssinger Nicolas 769664 896-903 10.1039/C7SC02736B 2041-6520 http://xlink.rsc.org/?DOI=C7SC02736B The development of a rapid and chemoselective selenocystine–selenoester peptide ligation that operates at nanomolar reactant concentrations has been developed by utilising PNA templation. Kinetic analysis of the templated peptide ligation revealed that the selenocystine–selenoester reaction was 10 times faster than traditional native chemical ligation at cysteine and to our knowledge is the fastest templated ligation reaction reported to date. The efficiency and operational simplicity of this technology is highlighted through the formation of hairpin molecular architectures and in a novel paper-based lateral flow assay for the rapid and sequence specific detection of oligonucleotides, including miRNA in cell lysates. Dewey 540 36 Département de chimie organique 141 false 762 Groupe Winssinger Chemical Science 9 4 85d54813-792e-473b-a235-35ddf4d8471a Article (Published version) Public c7sc02736b.pdf 1214522 application/pdf CC BY-NC openAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie organique

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Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection	Chemical Science			2018	583	271

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Sayers, Jessica