Fast identification of dermatophytes by MALDI-TOF/MS using direct transfer of fungal cells on ground steel target plates

Article Article scientifique Article Fast identification of dermatophytes by MALDI-TOF/MS using direct transfer of fungal cells on ground steel target plates eng 2018 author Da Cunha Keith Cassia 842283 author Riat Arnaud 134645 author Normand Anne-Cecile author Bosshard Philipp P author de Almeida Margarete T G author Piarroux Renaud author Schrenzel Jacques 29184 author Fontao Lionel 514347 691-697 10.1111/myc.12793 29762871 0933-7407 Dermatophytes cause human infections limited to keratinised tissues. We showed that the direct transfer method allows reliable identification of non-dermatophytes mould and yeast by MALDI-TOF/MS. We aimed at assessing whether the direct transfer method can be used for dermatophytes and whether an own mass spectra library would be superior to the Bruker library. We used the Bruker Biotyper to build a dermatophyte mass spectra library and assessed its performance by 1/testing a panel of mass spectrum produced with strains genotypically identified and, 2/comparing MALDI-TOF/MS identification to morphology-based methods. Identification of dermatophytes using the Bruker library is poor. Our library provided 97% concordance between ITS sequencing and MALDI-TOF/MS analysis with a panel of 1104 spectra corresponding to 276 strains. Direct transfer method using unpolished target plates allowed proper identification of 85% of dermatophytes clinical isolates most of which were common dermatophytes. A homemade dermatophyte MSP library is a prerequisite for accurate identification of species absent in the Bruker library but it also improves identification of species already listed in the database. The direct deposit method can be used to identify the most commonly found dermatophytes such as T. rubrum and T. interdigitale/mentagrophytes by MALDI-TOF/MS. Arthrodermataceae/chemistry/classification/isolation & purification Dermatomycoses/diagnosis/microbiology Humans Microbiological Techniques/methods Specimen Handling/methods Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization/methods Dewey 616 56 Département de médecine 221 false 338 Génomique et microbiote 604 1331 Syphilis 1023 576 Inflammation cutanée 919 Mycoses 61 9 a1e3b207-57bb-4676-9cea-b5f23e7ca6ec Article (Published version) Restricted myc.12793.pdf 681708 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine

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Fast identification of dermatophytes by MALDI-TOF/MS using direct transfer of fungal cells on ground steel target plates	Mycoses			2018	361	1

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Da Cunha, Keith Cassia