Breaking the Asynchrony: Importance of Cell Division Timing on Cell Fate

Diplôme Master Breaking the Asynchrony: Importance of Cell Division Timing on Cell Fate eng 2017 author Monteiro Pearl 835654 director Gotta Monica 0000-0002-4336-7860 659510 During development, the length of each cell cycle is tightly regulated. The cell cycle timing has an impact on cell fate and ultimately the development of the whole organism. However, how changes in cell cycle duration control cell fate remains elusive. In C. elegans embryo cell cycle asynchrony is very robust, suggesting that it is important for embryonic development. However, the consequences of losing this asynchrony on cell fate and development of embryos are not known. Here we study a viable mutant of MEX-5 in which PLK-1 remains asymmetrically localized. After depletion of mex-6 these mutant embryos are unable to drive anterior localization of PLK-1, divide synchronously at two cell stage, regain asynchrony at four cell stage and are embryonic lethal, PIE-1 is symmetrically localized at two cell stage and shows dynamic localization patterns at later stages. We observe normal establishment of upstream PAR polarity. Dewey 570 Dewey 612 Genetics Development and Evolution 43 Département de biologie moléculaire 161 134 Département de physiologie cellulaire et métabolisme 21C false b58ca02b-f319-4898-be43-bd4212d89c7a Master thesis Private MasterThesis(PMonteiro).pdf 15534320 application/pdf closedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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Breaking the Asynchrony: Importance of Cell Division Timing on Cell Fate				2017	111	0

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Monteiro, Pearl