Monomers and dimers of the RepA protein in plasmid pSC101 replication: domains in RepA

Article Article scientifique Article Monomers and dimers of the RepA protein in plasmid pSC101 replication: domains in RepA eng 1992 author Manen-Commandeur Danielle 22369 author Upegui-Gonzalez Lia Cristina 83495 author Caro Lucien 25084 8923-8927 10.1073/pnas.89.19.8923 0027-8424 http://www.pnas.org/cgi/doi/10.1073/pnas.89.19.8923 The replication of plasmid pSC101 requires the plasmid-encoded protein RepA. This protein has a double role: it binds to three directly repeated sequences in the pSC101 origin and promotes replication of the plasmid; it binds to two inversely repeated sequences in its promoter region and regulates its own transcription. A series of RepA protein derivatives carrying deletions of the C-terminal region were assayed for specific binding. We found that the last third of the protein is not needed for binding to the various specific sites. Truncated proteins that still bind can also form heterodimers with a wild-type protein. Analysis of band retardation assays conducted with wild-type and truncated proteins indicates that RepA binds to directly repeated sequences as a monomer and to inversely repeated sequences as a dimer. Communicated by Werner Arber DNA replication DNA-binding protein Binding sites Dewey 570 Swiss National Science Foundation 31-25698.88 CH 43 Département de biologie moléculaire 161 false Proceedings of the National Academy of Sciences 89 19 5e11d7d1-cebf-4f07-b916-4060122da45c Article (Published version) Public 8923.full.pdf 1210824 application/pdf openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Monomers and dimers of the RepA protein in plasmid pSC101 replication: domains in RepA	Proceedings of the National Academy of Sciences			1992	216	197

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Upegui-Gonzalez, Lia Cristina