A translaminar genetic logic for the circuit identity of intracortically projecting neurons

Article Article scientifique Article A translaminar genetic logic for the circuit identity of intracortically projecting neurons eng 2019 author Klingler Esther 831648 author De La Rossa Andrés 642868 author Fievre Sabine 850352 author Devaraju Karthikeyan 831125 author Abe Philipp 889742 author Jabaudon Denis 0000-0003-2438-4769 682473 332-339.e5 10.1016/j.cub.2018.11.071 30639110 0960-9822 https://www.sciencedirect.com/science/article/pii/S0960982218316014 Neurons of the neocortex are organized into six radial layers, which have appeared at different times during evolution, with the superficial layers representing a more recent acquisition. Input to the neocortex predominantly reaches superficial layers (SL, i.e., layers (L) 2-4), while output is generated in deep layers (DL, i.e., L5-6) [1]. Intracortical connections, which bridge input and output pathways, are key components of cortical circuits because they allow the propagation and processing of information within the neocortex. Two main types of intracortically projecting neurons (ICPN) can be distinguished by their axonal features: L4 spiny stellate neurons (SSN) with short axons projecting locally within cortical columns [2-5], and SL and DL long-range projection neurons, including callosally projecting neurons (CPNSL and CPNDL) [5, 6]. Here, we investigate the molecular hallmarks that distinguish SSN, CPNSL, and CPNDL and relate their transcriptional signatures with their output connectivity. Specifically, taking advantage of the presence of CPN in both SL and DL, we identify lamina-independent genetic hallmarks of a constant projection motif (i.e., interhemispheric projection). By performing unbiased transcriptomic comparisons between CPNSL, CPNDL and SSN, we provide specific molecular profiles for each of these populations and show that target identity supersedes laminar position in defining ICPN transcriptional diversity. Together, these findings reveal a projection-based organization of transcriptional programs across cortical layers, which we propose reflects conserved strategy to protect canonical circuit structure (and hence function) across a diverse range of neuroanatomies. Dewey 616.8 53 Département de neurosciences fondamentales 21A 61 Département des neurosciences cliniques 226 false 540 Neurobiologie développementale et plasticité 880 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE122742 Current Biology 29 2 81ee5221-06d0-4a8f-9d01-37641f4cf5bf Article (Published version) Restricted 1-s2.0-S0960982218316014-main.pdf 4491611 application/pdf Vignette Public Vignette_1-s2.0-S0960982218316014-fx1.jpg 67729 image/jpeg restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques

Title	Published in	Access level	OA Policy	Year	Views	Downloads

A translaminar genetic logic for the circuit identity of intracortically projecting neurons	Current Biology			2019	632	14

Archive ouverte UNIGE

Devaraju, Karthikeyan