Co-translational assembly of proteasome subunits in NOT1-containing assemblysomes

Article Article scientifique Article Co-translational assembly of proteasome subunits in NOT1-containing assemblysomes eng 2019 author Panasenko Olesya 629191 author Somasekharan Syam Prakash 650362 author Villanyi Zoltan 766412 author Zagatti Marina 885449 author Bezrukov Fedor 849834 author Rashpa Ravish 844040 author Cornut Julien 696098 author Iqbal Jawad 826762 author Longis Marion 873336 author Carl Sarah H author Peña Cohue author Panse Vikram G author Collart Martine 0000-0001-6955-1773 27009 110-120 10.1038/s41594-018-0179-5 30692646 1545-9985 The assembly of large multimeric complexes in the crowded cytoplasm is challenging. Here we reveal a mechanism that ensures accurate production of the yeast proteasome, involving ribosome pausing and co-translational assembly of Rpt1 and Rpt2. Interaction of nascent Rpt1 and Rpt2 then lifts ribosome pausing. We show that the N-terminal disordered domain of Rpt1 is required to ensure efficient ribosome pausing and association of nascent Rpt1 protein complexes into heavy particles, wherein the nascent protein complexes escape ribosome quality control. Immunofluorescence and in situ hybridization studies indicate that Rpt1- and Rpt2-encoding messenger RNAs co-localize in these particles that contain, and are dependent on, Not1, the scaffold of the Ccr4-Not complex. We refer to these particles as Not1-containing assemblysomes, as they are smaller than and distinct from other RNA granules such as stress granules and GW- or P-bodies. Synthesis of Rpt1 with ribosome pausing and Not1-containing assemblysome induction is conserved from yeast to human cells. Dewey 616 Swiss National Science Foundation Genetic and biochemical analysis of the regulation of gene expression by the Ccr4-Not complex in the yeast S.cerevisiae 135794 CH Projects Swiss National Science Foundation Genetic and biochemical analysis of the regulation of gene expression by the Ccr4-Not complex in the yeast S.cerevisiae 172999 CH Projects Swiss National Science Foundation Exploring the interface between ribosome assembly and nucleocytoplasmic transport 166571 CH Projects 54 Département de microbiologie et médecine moléculaire 21B false 58 Caractérisation génétique et biochimique du complexe Ccr4-Not conservé. 81 Nature Structural & Molecular Biology 26 2 42387ecd-40ea-4899-907f-10dff7409d2c Article (Published version) Restricted s41594-018-0179-5(1).pdf 3740079 application/pdf 482a0980-3b97-442d-8962-2d26d6b18437 Article (Accepted version) Public collart-edver_1543943588.pdf 514027 application/pdf f5cafca0-37fb-4ac5-89bb-31d05cb8caba Appendix Restricted 41594_2018_179_MOESM1_ESM.pdf 117029616 application/pdf 44a0fd21-412f-4d5a-83e8-8e1ac2c725b8 Supplemental data Restricted Supplementary_Table_1_copy.xlsx 101256 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 72523a95-ad9b-4d23-9a96-afbec495ea90 Supplemental data Restricted Supplementary_Table_2_copy.xlsx 14872 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Co-translational assembly of proteasome subunits in NOT1-containing assemblysomes	Nature Structural & Molecular Biology			2019	845	779

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Iqbal, Jawad