Effect of the nonspecific binding in differential impedance biosensing

Article Article scientifique Article Effect of the nonspecific binding in differential impedance biosensing eng 2019 author Buff Mathias Quentin Patrice 0009-0005-2338-8954 825407 author Drab Ewa Beata 857097 Ewa Beata Grassin author Sugihara Kaori 818285 011004 10.1116/1.5082717 1559-4106 http://avs.scitation.org/doi/10.1116/1.5082717 The detection limits of impedance biosensors are dictated by the presence of background nonspecific binding, yet almost all the detection limits reported in the literature are determined using a clean buffer solution without confronting this real challenge. In this work, the authors employed the simplest “differential” impedance biosensor, composed of poly-l-lysine-polyethylene glycol-biotin-coated gold electrodes for the detection of streptavidin in the presence of 0.1% fetal calf serum, and studied the effect of the nonspecific binding on the performance of the differential impedance biosensing. The lowest streptavidin concentration detected by the system (5 μg/ml) was 1 order of magnitude higher (worse) than that from a previously demonstrated impedance biosensor where avidin was detected in the absence of background proteins. Interestingly, the origin of the differential signal was not due to the electrochemical properties of streptavidin itself but was that of the serum, where the coverage of the electrode by streptavidin indirectly modulated the electrical signal by suppressing the accessibility of the serum to the electrode. Dewey 540 38 Département de chimie physique 142 false Biointerphases 14 1 1ac01703-0080-4f51-badc-5e2134470dbc Article (Published version) Public Effect_KS_19.pdf 2701222 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie physique

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Effect of the nonspecific binding in differential impedance biosensing	Biointerphases			2019	263	231

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Buff, Mathias Quentin Patrice

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