Metabolomic- and molecular networking-based exploration of the chemical responses induced in citrus sinensis leaves inoculated with xanthomonas citri

Article Article scientifique Article Metabolomic- and molecular networking-based exploration of the chemical responses induced in citrus sinensis leaves inoculated with xanthomonas citri eng 2022-11-09 2022-11-23 author Saldanha Luiz Léonardo 0000-0003-2454-067X 824081 author Allard Pierre-Marie 0000-0003-3389-2191 812151 author Dilarri Guilherme author Codesido Sanchez Santiago 812906 author Gonzalez Ruiz Victor 0000-0001-7204-2363 831974 author Ferreira Queiroz Emerson 0000-0001-9567-1664 135675 author Ferreira Henrique 0000-0002-9183-9420 author Wolfender Jean-Luc 0000-0002-0125-952X 135649 14693-14705 10.1021/acs.jafc.2c05156 36350271 0021-8561 https://pubs.acs.org/doi/abs/10.1021/acs.jafc.2c05156 Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (X. citri), is a plant disease affecting Citrus crops worldwide. However, little is known about defense compounds in Citrus. Here, we conducted a mass spectrometry-based metabolomic approach to obtain an overview of the chemical responses of Citrus leaves to X. citri infection. To facilitate result interpretation, the multivariate analyses were combined with molecular networking to identify biomarkers. Metabolite variations among untreated and X. citri-inoculated Citrus samples under greenhouse conditions highlighted induced defense biomarkers. Notably, the plant tryptophan metabolism pathway was activated, leading to the accumulation of N-methylated tryptamine derivatives. This finding was subsequently confirmed in symptomatic leaves in the field. Several tryptamine derivatives showed inhibitory effects in vitro against X. citri. This approach has enabled the identification of new chemically related biomarker groups and their dynamics in the response of Citrus leaves to Xanthomonas infection. Biotic stress Citrus canker Pathogen-induced metabolites Sweet orange Tryptamines Dewey 615 Switzerland FAPESP BEPE2019/00307-5 31 Section des sciences pharmaceutiques 17 false 881 Sciences analytiques Journal of agricultural and food chemistry 70 46 7e54360c-de0e-4cb8-8c9a-0c922f378c24 Article (Published version) Restricted Article-scientifique-from-crossref.pdf 6546286 application/pdf Vignette Public images_large_jf2c05156_0009.jpeg 190036 image/jpeg restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Metabolomic- and molecular networking-based exploration of the chemical responses induced in citrus sinensis leaves inoculated with xanthomonas citri	Journal of agricultural and food chemistry			2022	166	0

Archive ouverte UNIGE

Saldanha, Luiz Léonardo