Quantitative CE analysis of punicalagin in <i>Combretum aculeatum</i> extracts traditionally used in Senegal for the treatment of tuberculosis

Article Article scientifique Article Quantitative CE analysis of punicalagin in <i>Combretum aculeatum</i> extracts traditionally used in Senegal for the treatment of tuberculosis fre 2019 author Diop El Hadji Assane 558013 author Jacquat Jenna 823732 author Drouin Nicolas 808691 author Ferreira Queiroz Emerson 135675 author Wolfender Jean-Luc 135649 author Diop Tahir author Schappler Julie 506604 author Rudaz Serge 0000-0002-4180-5417 28978 2820-2827 10.1002/elps.201900240 31407800 0173-0835 https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900240 Mycobacterium tuberculosis is the causative agent of tuberculosis, an infectious bacterial disease, which most commonly affects the lungs. In the search for novel active compounds or medicines against tuberculosis, an ethnopharmacological survey combined with a host‐pathogen assay has recently highlighted the potency of an aqueous extract of Combretum aculeatum. C. aculeatum is used in traditional medicine and has demonstrated a significant in vitro antimycobacterial activity. Punicalagin, an ellagitannin, was isolated and found to be related to the biological activity of the extract. An analytical method for the evaluation of punicalagin in C. aculeatum was developed by capillary electrophoresis. After method optimization, the quantification of punicalagin was achieved for the evaluation of various plant extracts to determine the content of punicalagin related to the extraction modes and conditions, origin of the plant material, and harvesting period. The developed method demonstrated that the leaves presented the highest punicalagin content compared to the seeds and stems. A decoction of 30 min in boiling water was found to be the best extraction mode of C. aculeatum. Capillary electrophoresis Combretum aculeatum Method development Punicalagin Tuberculosis Dewey 615 31 Section des sciences pharmaceutiques 17 false 881 Sciences analytiques Electrophoresis 40 21 62bf1ed1-777e-4ddb-947a-ecef1c2a6c45 Article (Published version) Restricted Quantitative_CE_analysis_of_punicalagin_in_Combretum_aculeatum_extracts_traditionally_used_in_Senegal_for_the_treatment_of_tuberculosis.pdf 534967 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Year	Views	Downloads

Quantitative CE analysis of punicalagin in Combretum aculeatum extracts traditionally used in Senegal for the treatment of tuberculosis	Electrophoresis	2019	328	0
Dosage de la punicalagine par électrophorèse capillaire dans des extraits végétaux utilisés en médecine traditionnelle contre la tuberculose		2017	104	2

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Jacquat, Jenna