Study of diabetic bone fragility in PEDF-deficient mice

Diplôme Master Study of diabetic bone fragility in PEDF-deficient mice eng 2021-09-16 author Li Hoi Ting Viviane 823051 collaborator Gerbaix Maude Coline 790754 director Ferrari Serge Livio 24314 In this work, we studied the effects of the PEDF gene on lipid and carbohydrate metabolism and bone microarchitecture in a mouse model. The pigment epithelium derived factor (PEDF), also known as SERPINF1, is a 50 kDa glycoprotein of the serine proteinase inhibitor (SERPIN) superfamily. It has been observed that this gene is responsible for bone diseases such as osteogenesis imperfecta type VI and that the expression of this gene is increased in serum in type 2 diabetic patients, which have an increased risk of bone fracture. We investigated whether there is a link between the PEDF gene and the increased risk of bone fracture in a diabetic context. My work consists of studying this relationship, using PEDF KO mice and a high fat diet (HFD), which induces impaired glucose tolerance and impacts the skeleton. I showed that the absence of PEDF reduced the number and connectivity of trabeculae and increased the density of bone tissue. My results showed that the high-fat diet had an impact on the metaphyseal cortex of the femur and on lipid and carbohydrate metabolism. Dewey 590 44 Département de génétique et évolution 162 false cd7f43f6-a4f0-465e-ac01-3f17a19b99d1 Master thesis Restricted 2021_09_28_Master_VivianeLI.pdf 4754200 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Study of diabetic bone fragility in PEDF-deficient mice				2021	101	2

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Li, Hoi Ting Viviane