HBx and the Smc5/6 restriction factor

Diplôme Thèse HBx and the Smc5/6 restriction factor eng 2020-05-15 author Zotter Lisa Maria 821576 director Strubin Michel 22429 10.13097/archive-ouverte/unige:142468 urn:nbn:ch:unige-1424682 Sc. Vie 56 Despite the availability of a vaccine, chronic hepatitis B virus (HBV) infection remains a leading cause of cirrhosis and liver cancer. The genome of HBV persists as an extrachromosomal DNA circle in infected cells. The family of Hepadnaviridae includes mammalian and avian hepatitis B viruses. Mammalian HBV genomes, but not the avian HBV genomes, encode an X protein that causes degradation of the multi-subunit restriction factor Smc5/6. The Smc5/6 complex can specifically associate with and block transcription from extrachromosomal DNA. Experiments in chicken cells suggest that none of the X proteins tested cause degradation of the avian Smc5/6 complex and that the avian complex lacks restriction activity. These findings where exploited to perform cross-species experiments to identify subunit(s) involved in Smc5/6 restriction activity and/or binding to the Xproteins. The results so far suggest that one chicken Smc5/6 subunit, Nse4, lacks a function essential for the restriction activity. Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false d4d92684-089b-417a-a0e7-10f60817128c Thesis Public Zotter_Thesis.pdf 3804922 application/pdf Private 2025-09-28 7294e48d22ae9c5f4bea2ea4c6f6b9105af00102b761c28173b0955fafb62f75 openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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HBx and the Smc5/6 restriction factor				2020	710	382

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Zotter, Lisa Maria