The Staphylococcus aureus Chaperone PrsA Is a New Auxiliary Factor of Oxacillin Resistance Affecting Penicillin-Binding Protein 2A

Article Article scientifique Article The Staphylococcus aureus Chaperone PrsA Is a New Auxiliary Factor of Oxacillin Resistance Affecting Penicillin-Binding Protein 2A eng 2016 author Jousselin Ambre 715999 author Manzano Caroline Stéphanie 89052 author Biette Alexandra 821529 author Reed Patricia author Pinho Mariana G author Rosato Adriana E author Kelley William L author Renzoni Adriana Maria 134435 1656-1666 10.1128/AAC.02333-15 26711778 0066-4804 Expression of the methicillin-resistant S. aureus (MRSA) phenotype results from the expression of the extra penicillin-binding protein 2A (PBP2A), which is encoded by mecA and acquired horizontally on part of the SCCmec cassette. PBP2A can catalyze dd-transpeptidation of peptidoglycan (PG) because of its low affinity for β-lactam antibiotics and can functionally cooperate with the PBP2 transglycosylase in the biosynthesis of PG. Here, we focus upon the role of the membrane-bound PrsA foldase protein as a regulator of β-lactam resistance expression. Deletion of prsA altered oxacillin resistance in three different SCCmec backgrounds and, more importantly, caused a decrease in PBP2A membrane amounts without affecting mecA mRNA levels. The N- and C-terminal domains of PrsA were found to be critical features for PBP2A protein membrane levels and oxacillin resistance. We propose that PrsA has a role in posttranscriptional maturation of PBP2A, possibly in the export and/or folding of newly synthesized PBP2A. This additional level of control in the expression of the mecA-dependent MRSA phenotype constitutes an opportunity to expand the strategies to design anti-infective agents. Dewey 612 Dewey 616 134 Département de physiologie cellulaire et métabolisme 21C 56 Département de médecine 221 false 63 Acquisition et expression de facteurs de virulence chez Staphylococcus aureus 86 Antimicrobial agents and chemotherapy 60 3 bb7cbf2d-6dbf-43b0-a5fd-477c30f69689 Article (Published version) Restricted 21656.full.pdf 1080688 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme Faculté de médecine / Section de médecine clinique / Département de médecine

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The Staphylococcus aureus Chaperone PrsA Is a New Auxiliary Factor of Oxacillin Resistance Affecting Penicillin-Binding Protein 2A	Antimicrobial agents and chemotherapy			2016	618	0

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Biette, Alexandra