The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus

Article Article scientifique Article The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus eng 2020-05-05 2020-05-05 author Noordally Zeenat 0000-0003-2817-1330 Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 854962 author Trichtinger Celso Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 819313 author Dalvit Ivan Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 672527 Département des sciences végétales (BIVEG) author Hofmann Manuel Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 813220 author Roux Céline Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 701213 Département des sciences végétales (BIVEG) author Zamboni Nicola 0000-0003-1271-1021 Institute of Molecular Systems Biology, ETH Zurich, 8093, Zurich, Switzerland. author Pourcel Lucille Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 728803 author Gas Pascual Elisabet 0000-0002-2643-6916 Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 744148 author Gisler Alexandra Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. 686734 author Fitzpatrick Thérésa Bridget 0000-0001-7694-5631 Department of Botany and Plant Biology, University of Geneva, 1211, Geneva, Switzerland. theresa.fitzpatrick@unige.ch. 684438 Département des sciences végétales (BIVEG) 209 10.1038/s42003-020-0927-z 32372067 2399-3642 PMC7200797 In plants, metabolic homeostasis-the driving force of growth and development-is achieved through the dynamic behavior of a network of enzymes, many of which depend on coenzymes for activity. The circadian clock is established to influence coordination of supply and demand of metabolites. Metabolic oscillations independent of the circadian clock, particularly at the subcellular level is unexplored. Here, we reveal a metabolic rhythm of the essential coenzyme thiamine diphosphate (TDP) in the Arabidopsis nucleus. We show there is temporal separation of the clock control of cellular biosynthesis and transport of TDP at the transcriptional level. Taking advantage of the sole reported riboswitch metabolite sensor in plants, we show that TDP oscillates in the nucleus. This oscillation is a function of a light-dark cycle and is independent of circadian clock control. The findings are important to understand plant fitness in terms of metabolite rhythms. Arabidopsis / metabolism Cell Nucleus / metabolism Circadian Rhythm Photoperiod Thiamine Pyrophosphate / metabolism Dewey 580 Swiss National Science Foundation Unraveling the molecular mechanisms and regulatory networks controlling responses to thiamin (vitamin B1) or pyridoxine (vitamin B6) in Arabidopsis. 141117 CH Projects Swiss National Science Foundation Dissecting the role of vitamin B1 derivatives and the interaction between vitamin B1 homeostasis and the circadian clock. 162555 CH Projects 45 Département des sciences végétales 163 false Communications biology 3 1 b8c31cab-7f36-499f-8732-26997870ce3b Appendix Public 42003_2020_927_MOESM2_ESM.xlsx 93032 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 3c15f540b9be050d6d9a17d9625e4b961c7bd8798574ef5e96e6a8007c613dff 7f6e682e-64cb-40e7-bd28-cda035a40009 Appendix Public 42003_2020_927_MOESM4_ESM.pdf 30289 application/pdf 59f302122c825fbc96f5ed148fa0ff613669a915ea9f3705e87f137d1362d1e8 9b78cffc-996b-4819-9d84-998cedae5094 Appendix Public 42003_2020_927_MOESM1_ESM.pdf 4172295 application/pdf 490ba47b833eec1a015320c046a882dca452a1641d0100d3de6b3d95f983beb2 115bf9de-7e37-4457-8a67-c7bc2cb03719 Appendix Public 42003_2020_927_MOESM3_ESM.pdf 827277 application/pdf 7d83078b314465a7825bf4d4ca2ccecb8175d39e67999735b905869190e19c42 e9dbc512-f4fe-4e1f-84b6-c1d0a59c3e9f Article (Published version) Public Article-scientifique-from-europePmc.pdf 3941588 application/pdf CC BY 6c2371e6aa2c64ab18f82e39cde7e295bf0bf626ec09670a22b67b995c42d4d8 openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus	Communications biology			2020	103	339

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Trichtinger, Celso