Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy

Article Article scientifique Article Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy eng 2018 author Kishazi Edina 818253 author Dor Marianne 837522 author Eperon Simone author Oberic Aurélie author Turck Natacha 632628 author Hamedani Mehrad 10792 10.1038/s41598-018-29113-2 30018377 2045-2322 https://www.nature.com/articles/s41598-018-29113-2 The aim was to investigate the levels of cytokines and soluble IL-6R in the tears of patients with thyroid-associated orbitopathy (TAO) disease. Schirmer's test was adopted to collect tears from TAO patients (N = 20, 17 women, mean age (±SD): 46.0 years (±13.4)) and healthy subjects (N = 18, 10 women, 45.4 years (±18.7)). Lacrimal cytokines and soluble IL-6R (sIL-6R) were measured using a 10-plex panel (Meso Scale Discovery Company) and Invitrogen Human sIL-6R Elisa kit, respectively. Tear levels of IL-10, IL-12p70, IL-13, IL-6 and TNF-α appeared significantly higher in TAO patients than in healthy subjects. Interestingly, IL-10, IL-12p70 and IL-8 levels increased in tears whatever the form of TAO whereas IL-13, IL-6 and TNF-α levels were significantly elevated in inflammatory TAO patients, meaning with a clinical score activity (CAS) ≥ 3, compared to controls. Furthermore, only 3 cytokines were strongly positively correlated with CAS (IL-13 Spearman coeff. r: 0.703, p = 0.0005; IL-6 r: 0.553, p = 0.011; IL-8 r: 0.618, p = 0.004, respectively). Finally, tobacco use disturbed the levels of several cytokines, especially in patient suffering of TAO. The differential profile of lacrimal cytokines could be useful for the diagnosis of TAO patients. Nevertheless, the tobacco use of these patients should be taken into account in the interpretation of the cytokine levels. Biomarkers/metabolism Cytokines/metabolism Female Graves Ophthalmopathy/diagnosis/immunology/metabolism Humans Male Middle Aged Tears/metabolism Dewey 610 3 Faculté de médecine 2 false Scientific Reports 8 1 bc879a2a-fc49-4456-a2d6-a51ca594a051 Article (Published version) Public s41598-018-29113-2.pdf 2032147 application/pdf CC BY 4b6c15d9-e69e-4c16-ae6b-b344e6dd1559 Supplemental data Public 41598_2018_29113_MOESM1_ESM.pdf 389129 application/pdf openAccess Faculté de médecine

Title	Published in	Year	Views	Downloads

Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy	Scientific Reports	2018	313	226
Thyroid-associated orbitopathy and tears: A proteomics study	Journal of Proteomics	2018	551	12

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Kishazi, Edina