Effect of the Interaction of the Amyloid β (1–42) Peptide with Short Single-Stranded Synthetic Nucleotide Sequences: Morphological Characterization of the Inhibition of Fibrils Formation and Fibrils Disassembly

Article Article scientifique Article Effect of the Interaction of the Amyloid β (1–42) Peptide with Short Single-Stranded Synthetic Nucleotide Sequences: Morphological Characterization of the Inhibition of Fibrils Formation and Fibrils Disassembly eng 2014 author Abraham Jancy 781623 author Kedracki Dawid 748449 author Prado Enora 817570 author Gourmel Charlotte 633588 author Maroni Plinio 644101 author Nardin Corinne 739494 Corinne Vebert-Nardin 3253-3258 10.1021/bm501004q 1525-7797 The formation of extracellular neuritic plaques in the brain of individuals who suffered from Alzheimer's disease (AD) is a major pathological hallmark. These plaques consist of filamentous aggregates of the amyloid beta (1–42) (Aβ42) proteins. Prevention or reduction of the formation of these fibrils is foreseen as a potential therapeutic approach. In this context, we investigated the interactions between the Aβ42 protein and polyions, in particular short single stranded synthetic nucleotide sequences. The experimental outcomes reported herein provide evidence of the inhibition of amyloid fibril genesis as well as disassembly of existing fibers through electrostatic interaction between the Aβ42 protein and the polyions. Since the polyions and the Aβ42 protein are oppositely charged, the formation of (micellar) inter polyelectrolyte complexes (IPECs) is likely to occur. Since the abnormal deposition of amyloid fibers is an archetype of AD, the outcomes of these investigations, supported by atomic force microscopy imaging in the dry and liquid states, as well as circular dichroism and Fourier transform infrared spectroscopy, are of high interest for the development of future strategies to cure a disease that concerns an ever aging population. Dewey 540 40 Département de chimie minérale, analytique et appliquée 145 false Biomacromolecules 15 9 034ac71e-9528-4290-92e7-68b1270efa3c Article (Published version) Restricted Corinne Nardin 0914 B.pdf 4642391 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie minérale, analytique et appliquée

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Effect of the Interaction of the Amyloid β (1–42) Peptide with Short Single-Stranded Synthetic Nucleotide Sequences: Morphological Characterization of the Inhibition of Fibrils Formation and Fibrils Disassembly	Biomacromolecules			2014	719	2

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