HP1 protein Chp2 selectively recruits nucleosome remodeler through non-canonical interaction

Diplôme Thèse HP1 protein Chp2 selectively recruits nucleosome remodeler through non-canonical interaction eng 2018-04-28 author Leopold Karoline 813337 director Schalch Thomas 787942 10.13097/archive-ouverte/unige:104618 urn:nbn:ch:unige-1046181 Sc. 5206 The establishment and maintenance of heterochromatic regions within the genome is an important factor for chromosome stability and con- trolled gene expression. It is dependent on many protein factors that are highly conserved from yeast to human. Among them are HP1 pro- teins that recognize heterochromatin-specific methylation marks and are involved in the recruitment of effector proteins. In my thesis, I will focus on the fission yeast HP1 protein Chp2 and its interaction with the Snf2/HDAC- containing remodeling complex SHREC, a homolog of NuRD complexes in higher organisms. Previously, Chp2 was found to be functionally and biochemically associated with the chromatin remodeler Mit1, a subunit of the SHREC complex. However, details of this interaction were unknown. I was able to solve the structure of the Chp2-Mit1 complex with high resolution and could show that an extensive interface between the two proteins provides high-affinity binding. The data reveals an unusual mode of HP1-client interaction and provides an example how specificity between different HP1 proteins canbe achieved. My work adds to the current knowledge about the highly conserved class of HP1 proteins and deepens our understanding of the molecular basis of their function. HP1 proteins Chromatin remodeler Structural biology Dewey 570 43 Département de biologie moléculaire 161 false 815 Groupe Schalch 0f61fe3a-e936-44ba-8398-9a785d135d88 Thesis Public Thesisfinal.pdf 13594404 application/pdf Private 2018-11-18 e3964819c575e4afdf7148fb1cd2f404642dd63effacf096d8dacc44bf2ae768 openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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HP1 protein Chp2 selectively recruits nucleosome remodeler through non-canonical interaction				2018	553	325

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Leopold, Karoline