CO2 assimilation in the chemocline of Lake Cadagno is dominated by a few types of phototrophic purple sulfur bacteria

Article Article scientifique Article CO2 assimilation in the chemocline of Lake Cadagno is dominated by a few types of phototrophic purple sulfur bacteria eng 2013-02-11 2013-05-11 author Storelli Nicola 687575 author Peduzzi Sandro Alpine Biology Center (ABC); Quinto; Switzerland 748595 Forel Communs (FOREL) author Saad Maged Department of Botany and Plant Biology, Microbiology Unit; University of Geneva, Sciences III; Geneva; Switzerland 517004 author Frigaard Niels-Ulrik Section for Marine Biology, Department of Biology; University of Copenhagen; Helsingør; Denmark author Perret Xavier Department of Botany and Plant Biology, Microbiology Unit; University of Geneva, Sciences III; Geneva; Switzerland 808713 author Tonolla Mauro Amedeo 45173 Département des sciences végétales (BIVEG) 421-432 10.1111/1574-6941.12074 23330958 0168-6496 https://academic.oup.com/femsec/article-lookup/doi/10.1111/1574-6941.12074 Lake Cadagno is characterized by a compact chemocline that harbors high concentrations of various phototrophic sulfur bacteria. Four strains representing the numerically most abundant populations in the chemocline were tested in dialysis bags in situ for their ability to fix CO₂. The purple sulfur bacterium Candidatus 'Thiodictyon syntrophicum' strain Cad16(T) had the highest CO₂ assimilation rate in the light of the four strains tested and had a high CO₂ assimilation rate even in the dark. The CO₂ assimilation of the population represented by strain Cad16(T) was estimated to be up to 25% of the total primary production in the chemocline. Pure cultures of strain Cad16(T) exposed to cycles of 12 h of light and 12 h of darkness exhibited the highest CO₂ assimilation during the first 4 h of light. The draft genome sequence of Cad16(T) showed the presence of cbbL and cbbM genes, which encode form I and form II of RuBisCO, respectively. Transcription analyses confirmed that, whereas cbbM remained poorly expressed throughout light and dark exposure, cbbL expression varied during the light-dark cycle and was affected by the available carbon sources. Interestingly, the peaks in cbbL expression did not correlate with the peaks in CO₂ assimilation. Primary production RuBisCO CbbL and cbbM mRNA Dewey 580 45 Département des sciences végétales 163 false FEMS microbiology ecology 84 2 7a0b20aa-bc93-43e5-a312-ee605692f4ca Article (Published version) Public Article_scientifique_from_unpaywall.pdf 300878 application/pdf e108666e4d3e5ae4a35e330d44c028dcca49f22dc90edd504dd349a8cbb59ac1 openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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CO₂ assimilation in the chemocline of Lake Cadagno is dominated by a few types of phototrophic purple sulfur bacteria	FEMS microbiology ecology			2013	60	264

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Perret, Xavier