Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

Article Article scientifique Article Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids eng 2021-11-23 2021-11-23 author Gendre Dylan Adrien Jason 774927 author Ameti Edis 808537 author Karenovics Wolfram 501317 author Perriraz-Mayer Nadja 89157 author Triponez Frédéric 28673 author Serre Beinier Véronique 0000-0002-6016-1645 123229 2375-2387 10.18632/oncotarget.28134 34853659 1949-2553 PMC8629400 https://www.oncotarget.com/article/28134/text/ Advanced lung cancers and mesothelioma remain incurable diseases. Despite some promising new therapy strategies, predicting whether an individual patient will be sensitive to a given therapy is challenging. The purpose of this study is to establish and evaluate the efficiency of a three-dimensional spheroid model of human thoracic cancer in predicting the efficacy of drugs. Human mesothelioma and lung tumor spheroids were established from cell lines and primary cells derived from the patient. The growth kinetics and cell viability of microtumors were assessed using spheroid size and intracellular ATP level. The sensitivity of the mesothelioma spheroids to the cisplatin or cisplatin/pemetrexed combination was determined. We determined that studying the kinetics of the spheroid growth for 15 days after seeding 1000 cells/well in a 96-well plate was optimal. Monitoring the growth kinetic and intracellular ATP of spheroids allowed the identification of early changes in spheroid viability. Finally, we validated this model by measuring a dose-dependent reduction in the cell viability of mesothelioma H2052/484 spheroids treated with both first-line treatments, cisplatin and the cisplatin/pemetrexed combination. In conclusion, we have developed a three-dimensional spheroid model of thoracic tumor cells useful for tailoring the medical treatment to the specific characteristics of each patient. Cancer patient Chemosensitivity Lung tumor Pleura mesothelioma Tumor spheroid Dewey 617 57 Département de chirurgie 222 false 902 Cancer du poumon et mésothéliome pleural malin 944 Oncotarget 12 24 9586b047-927c-4d39-a941-beb30ede53e8 Article (Published version) Public Article-scientifique-from-europePmc.pdf 2581227 application/pdf CC BY-3.0 0c5f0b3d-afef-4d0b-9fa3-3ba1f92aca36 Appendix Public oncotarget-12-2375-s001.pdf 1055027 application/pdf openAccess Faculté de médecine / Section de médecine clinique / Département de chirurgie

Title	Published in	Year	Views	Downloads

Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids	Oncotarget	2021	225	267
Characterization of isolated tumoral cell lines in monolayer and in spheroids for a reliable model in the study of lung adenocarcinoma		2019	150	2

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