Comparison of transplastomic Chlamydomonas reinhardtii and Nicotiana tabacum expression system for the production of a bacterial endoglucanase

Article Article scientifique Article Comparison of transplastomic Chlamydomonas reinhardtii and Nicotiana tabacum expression system for the production of a bacterial endoglucanase eng 2017 author Faè Matteo author Accossato Sonia 803504 author Cella Rino author Fontana Fabrizia 844629 author Goldschmidt-Clermont Michel P. 25594 author Leelavathi Sadhu author Reddy Vanga Siva author Longoni Paolo 797845 4085-4092 10.1007/s00253-017-8164-1 0175-7598 http://link.springer.com/10.1007/s00253-017-8164-1 The bulk production of recombinant enzymes by either prokaryotic or eukaryotic organisms might contribute to replace environmentally non-friendly chemistry-based industrial processes with enzyme-based biocatalysis, provided the cost of enzyme production is low. In this context, it is worth noting that the production of recombinant proteins by photosynthetic organisms offer both eukaryotic (nuclear) and prokaryotic (chloroplast) alternatives, along with the advantage of an autotrophic nutrition. Compared to nuclear transformation, chloroplast transformation generally allows a higher level of accumulation of the recombinant protein of interest. Furthermore, among the photosynthetic organisms, there is a choice of using either multicellular or unicellular ones. Tobacco, being a non-food and non-feed plant, has been considered as a good choice for producing enzymes with applications in technical industry, using a transplastomic approach. Also unicellular green algae, in particular Chlamydomonas reinhardtii, have been proposed as candidate organisms for the production of recombinant proteins. In the light of the different features of these two transplastomic systems, we decided to make a direct comparison of the efficiency of production of a bacterial endoglucanase. With respect to the amount obtained, 14 mg g-1 of biomass fresh weight equivalent to 8-10% of the total protein content, and estimated production cost, 1.5-2 € Kg-1, tobacco proved to be far more favourable for bulk enzyme production when compared to Chlamydomonas reinhardtii which accumulated this endoglucanase at 0.003% of the total protein. Chlamydomonas reinhardtii Tobacco Chloroplast Cellulase Molecular farming Dewey 580 Swiss National Science Foundation Chloroplast signaling and acclimation to changing light 146300 CH Project funding 45 Département des sciences végétales 163 false Applied Microbiology and Biotechnology 101 10 2b6db0c1-389f-418e-9289-e2e3c3d90402 Article (Accepted version) Restricted Fae_2017_revised.pdf 3039964 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Comparison of transplastomic Chlamydomonas reinhardtii and Nicotiana tabacum expression system for the production of a bacterial endoglucanase	Applied Microbiology and Biotechnology			2017	578	0

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