The Arabidopsis endosperm is a temperature-sensing tissue that implements seed thermoinhibition through phyB

Article Article scientifique Article The Arabidopsis endosperm is a temperature-sensing tissue that implements seed thermoinhibition through phyB eng 2023-03-07 2023-03-07 author Piskurewicz Urszula Department of Plant Sciences, University of Geneva, Geneva, Switzerland. 640565 Département des sciences végétales (BIVEG) author Sentandreu Maria 0000-0003-3626-5767 Department of Plant Sciences, University of Geneva, Geneva, Switzerland. 803105 author Iwasaki Mayumi 0000-0001-6561-4247 Department of Plant Sciences, University of Geneva, Geneva, Switzerland. 728942 Département des sciences végétales (BIVEG) author Glauser Gaëtan Neuchâtel Platform of Analytical Chemistry, Université de Neuchâtel, Neuchâtel, Switzerland. author Lopez Molina Luis 0000-0003-0463-1187 Department of Plant Sciences, University of Geneva, Geneva, Switzerland. Luis.LopezMolina@unige.ch.,Institute of Genetics and Genomics in Geneva (iGE3), University of Geneva, Geneva, Switzerland. Luis.LopezMolina@unige.ch. 28870 Département des sciences végétales (BIVEG) 1202 10.1038/s41467-023-36903-4 36882415 2041-1723 PMC9992654 Seed thermoinhibition, the repression of germination under high temperatures, prevents seedling establishment under potentially fatal conditions. Thermoinhibition is relevant for phenology and agriculture, particularly in a warming globe. The temperature sensing mechanisms and signaling pathways sustaining thermoinhibition are unknown. Here we show that thermoinhibition in Arabidopsis thaliana is not autonomously controlled by the embryo but is rather implemented by the endosperm. High temperature is sensed through endospermic phyB by accelerating its reversion from the active signaling Pfr form into the inactive Pr form, as previously described in seedlings. This leads to thermoinhibition mediated by PIFs, mainly PIF1, PIF3 and PIF5. Endospermic PIF3 represses the expression of the endospermic ABA catabolic gene CYP707A1 and promotes endospermic ABA accumulation and release towards the embryo to block its growth. Furthermore, endospermic ABA represses embryonic PIF3 accumulation that would otherwise promote embryonic growth. Hence, under high temperatures PIF3 exerts opposite growth responses in the endosperm and embryo. Agriculture Arabidopsis / genetics Arabidopsis Proteins / genetics Endosperm / genetics Phytochrome B / genetics Seedlings Seeds / genetics Temperature Basic Helix-Loop-Helix Transcription Factors / genetics Dewey 580 Swiss National Science Foundation Selected topics on the control of seed germination: light, temperature and dormancy 152660 CH Projects 45 Département des sciences végétales 163 false 831 Groupe Lopez-Molina Nature communications 14 1 dc40438f-f8fe-4403-8fa2-80ea61dff2f8 Appendix Public 41467_2023_36903_MOESM2_ESM.pdf 10810108 application/pdf 466949f20a9f2a65337aa7bb95798d683a4ac78d11b952fe8bdf4da8684cd04f 293a701a-6922-429a-916e-ee7f0b227a1e Appendix Public 41467_2023_36903_MOESM5_ESM.pdf 375971 application/pdf b8712ef8f942b46df5f68dd776fc6aa2d6148bdce1b1ab7677a4e8ae983abcc1 d7600db2-a716-4c39-a207-89aadb23ab2f Appendix Public 41467_2023_36903_MOESM1_ESM.pdf 5546407 application/pdf 2fcc07db7aa590d6502253f672d600a240d193997ebfc771ca165dfd90c12402 bb8d226e-8ba5-4b20-bee5-55dd04445607 Appendix Public 41467_2023_36903_MOESM3_ESM.pdf 112494 application/pdf 85eaa6663e2342a00d78377555bb1b4314bac763e204b9a66f935959065ed954 3a6a2890-62c5-44d5-bdf4-a9bf9f70a649 Appendix Public 41467_2023_36903_MOESM4_ESM.xlsx 8562534 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet fa450b478f7514250a544164f584bbf9e18a91a7c51edbe2f99097087d04497f e70a98b9-b04a-44fc-aa62-c375b50a4d9e Article (Published version) Public Article-scientifique-from-unpaywall.pdf 4295115 application/pdf CC BY e1e8090ba83e0bc4185ce22dbc58d82642329bea135694a699b13256bbbc1531 openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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The Arabidopsis endosperm is a temperature-sensing tissue that implements seed thermoinhibition through phyB	Nature communications			2023	139	634

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