Discovery and validation of tear biomarkers in orbitopathy disorders by proteomics

Diplôme Master Discovery and validation of tear biomarkers in orbitopathy disorders by proteomics eng 2015 author Gracia Maria De Los Angèles 802046 director Turck Natacha 632628 director Sanchez Jean-Charles 0000-0002-8733-5932 24399 director Martinou Jean-Claude 27293 Thyroid-Associated Orbitopathy (TAO) is an autoimmune disorder that affects extraocular muscles of the eye due to an accumulation of some factors e.g. Glycosaminoglycans. The aim is the identification of TAO biomarkers in tears to make easier the prediction and diagnosis and to increase the knowledge about TAO. Three independent 1D electrophoresis and isobaric Tandem Mass Tag (TMT) were analysed by Mass spectrometry, using EasyProt to obtain protein identification and quantification. Globally, combining the three experiments together, 567 tear proteins were quantified, with 221 differentially expressed between TAO and control patients. Pathways analyses revealed Glycolysis as the most representative pathway. 5 interesting dehydrogenases were found in this pathway and others. These results confirmed that tear fluid is a suitable source to research biomarkers for TAO disease. In addition, these proteins that are related and connected with the oxidative stress, could open new borderlines that could increase the general knowledge about TAO. Proteomic Biomarkers Tears Thyroid-Associated Orbitopathy Biological Fluid Dewey 570 Master en Biologie 30 Section de biologie 16 false 369 Biomarqueurs 635 8cf61802-68d6-402c-9794-5d8927d2eae0 Master thesis Restricted Master.pdf 5922161 application/pdf restrictedAccess Faculté des sciences / Section de biologie

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Discovery and validation of tear biomarkers in orbitopathy disorders by proteomics				2015	286	11

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Gracia, Maria De Los Angèles