Activation of the oncogenic miR-21-5p promotes HCV replication and steatosis induced by the viral core 3a protein

Article Article scientifique Article Activation of the oncogenic miR-21-5p promotes HCV replication and steatosis induced by the viral core 3a protein eng 2019 author Clement Sophie 759123 author Sobolewski Cyril 819918 author Rocha Gomes Diana Bianca 844324 author Rojas Alvarez-Ossorio Angela 800255 author Goossens Nicolas 509893 author Conzelmann Stéphanie 638487 author Calo Nicolas Virgile 643889 author Negro Francesco 96509 author Foti Michelangelo 0000-0001-7199-4135 28033 1226-1236 10.1111/liv.14112 30938910 1478-3223 miR-21-5p is a potent oncogenic microRNA targeting many key tumour suppressors including phosphatase and tensin homolog (PTEN). We recently identified PTEN as a key factor modulated by hepatitis C virus (HCV) to promote virion egress. In hepatocytes, expression of HCV-3a core protein was sufficient to downregulate PTEN and to trigger lipid droplet accumulation. Here, we investigated whether HCV controls PTEN expression through miR-21-5p-dependent mechanisms to trigger steatosis in hepatocytes and to promote HCV life cycle. Hepatitis C Lipid metabolism MicroRNA Phosphatase and tensin homolog Dewey 612 Dewey 616 Dewey 616.07 Swiss National Science Foundation Liver-derived circulating factors in intercellular/interorgan communication 172862 CH Project funding Swiss National Science Foundation Metabolic control of hepatocytes proliferation in regeneration and cancer. 160717 CH Programmes Swiss National Science Foundation 314730‐166609 CH 134 Département de physiologie cellulaire et métabolisme 21C 137 Département de pathologie et immunologie 21F 56 Département de médecine 221 false 297 Etudes et traitement de l'hépatite C et B 554 472 Obésité et syndrome métabolique 803 Liver International 39 7 f3448a9b-789e-41a1-bb66-a0cde8b5ef79 Article (Published version) Restricted Cl-ment_et_al-2019-Liver_International.pdf 1644981 application/pdf 3226f6f3-03c7-4622-8b9e-03548ceba443 Article (Accepted version) Public Clementetal_Liver Int_unpublished final version.pdf 2020863 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de médecine

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Activation of the oncogenic miR-21-5p promotes HCV replication and steatosis induced by the viral core 3a protein	Liver International			2019	457	299

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Rojas Alvarez-Ossorio, Angela