Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2

Article Article scientifique Article Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2 eng 2020 author Okuda Satohiro 835467 author Fujita Satoshi author Moretti Andréa 882215 author Hohmann Ulrich 823897 author Doblas Verónica G author Ma Yan author Pfister Alexandre author Brandt Benjamin 828267 author Geldner Niko 797544 author Hothorn Michael 826130 2693-2703 10.1073/pnas.1911553117 31964818 0027-8424 Plants use leucine-rich repeat receptor kinases (LRR-RKs) to sense sequence diverse peptide hormones at the cell surface. A 3.0-Å crystal structure of the LRR-RK GSO1/SGN3 regulating Casparian strip formation in the endodermis reveals a large spiral-shaped ectodomain. The domain provides a binding platform for 21 amino acid CIF peptide ligands, which are tyrosine sulfated by the tyrosylprotein sulfotransferase TPST/SGN2. GSO1/SGN3 harbors a binding pocket for sulfotyrosine and makes extended backbone interactions with CIF2. Quantitative biochemical comparisons reveal that GSO1/SGN3-CIF2 represents one of the strongest receptor-ligand pairs known in plants. Multiple missense mutations are required to block CIF2 binding in vitro and GSO1/SGN3 function in vivo. Using structure-guided sequence analysis we uncover previously uncharacterized CIF peptides conserved among higher plants. Quantitative binding assays with known and novel CIFs suggest that the homologous LRR-RKs GSO1/SGN3 and GSO2 have evolved unique peptide binding properties to control different developmental processes. A quantitative biochemical interaction screen, a CIF peptide antagonist and genetic analyses together implicate SERK proteins as essential coreceptor kinases required for GSO1/SGN3 and GSO2 receptor activation. Our work provides a mechanistic framework for the recognition of sequence-divergent peptide hormones in plants. Arabidopsis Receptor kinase Peptide hormone Root development Coreceptor Dewey 580 45 Département des sciences végétales 163 false 830 Groupe Hothorn Proceedings of the National Academy of Sciences 117 5 8f1ac2ed-65b1-4d6f-a045-aeec5ec4646e Article (Published version) Public 2693.full.pdf 3953692 application/pdf CC BY openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2	Proceedings of the National Academy of Sciences			2020	337	279

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Geldner, Niko