CRL4RBBP7 is required for efficient CENP-A deposition at centromeres

Article Article scientifique Article CRL4RBBP7 is required for efficient CENP-A deposition at centromeres eng 2015 author Mouysset Julien author Gilberto Samuel author Meier Michelle 795721 author Lampert Fabienne author Belwal Mukta author Meraldi Patrick 0000-0001-9742-8756 108140 author Peter Matthias 1732-1745 10.1242/jcs.162305 25795299 0021-9533 The mitotic spindle drives chromosome movement during mitosis and attaches to chromosomes at dedicated genomic loci named centromeres. Centromeres are epigenetically specified by their histone composition, namely the presence of the histone H3 variant CENP-A, which is regulated during the cell cycle by its dynamic expression and localization. Here, we combined biochemical methods and quantitative imaging approaches to investigate a new function of CUL4-RING E3 ubiquitin ligases (CRL4) in regulating CENP-A dynamics. We found that the core components CUL4 and DDB1 are required for centromeric loading of CENP-A, but do not influence CENP-A maintenance or pre-nucleosomal CENP-A levels. Interestingly, we identified RBBP7 as a substrate-specific CRL4 adaptor required for this process, in addition to its role in binding and stabilizing soluble CENP-A. Our data thus suggest that the CRL4 complex containing RBBP7 (CRL4(RBBP7)) might regulate mitosis by promoting ubiquitin-dependent loading of newly synthesized CENP-A during the G1 phase of the cell cycle. Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false 579 Groupe Meraldi Patrick (physiologie cellulaire et métabolisme) 922 Journal of cell science 128 9 bb254e6c-ef80-40bd-8328-a4d1edafb885 Article (Published version) Public J Cell Sci-2015-Mouysset-1732-45.pdf 2602038 application/pdf Restricted 2016-06-16 openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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CRL4RBBP7 is required for efficient CENP-A deposition at centromeres	Journal of cell science			2015	671	547

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Meier, Michelle