Immunological Characterization of Trichocyst Proteins in the Ciliate <i>Pseudomicrothorax dubius</i>

Article Article scientifique Article Immunological Characterization of Trichocyst Proteins in the Ciliate <i>Pseudomicrothorax dubius</i> eng 2007-05 1993 author Eperon Simone author Vigues Bernard Patrick 79567 author Peck Robert 25553 81-91 10.1111/j.1550-7408.1993.tb04886.x 8457804 1066-5234 Ejectable trichocysts were isolated from the ciliate Pseudomicrothorax dubius. Polyclonal antibodies were raised against three groups of trichocyst proteins: G1 (30‐31 kDa), G2 (26‐27 kDa) and G3 (15‐20 kDa). By indirect immunofluorescence, the three antisera strongly label the shafts of ejected trichocysts and the proximal ends of condensed trichocysts within the cells. By immunogold labeling for electron microscopy, the three sera specifically recognize the shafts of both extended and condensed trichocysts and shaft precursors in pretrichocysts as well. On one‐dimensional immunoblots of isolated trichocysts, anti‐G1 serum recognizes the G1 proteins, anti‐G2 serum detects G2 proteins and some G1 proteins, and anti‐G3 serum reacts with 15 bands, mainly the G3 and (30‐41)‐kDa proteins. In cells with and without trichocysts, the sera recognize non‐ejectable trichocyst proteins at 41‐42 kDa and 47 kDa. On two‐dimensional immunoblots of isolated trichocysts, anti‐G1 serum labels proteins with a pI of 4.75‐5.7, anti‐G2 serum labels proteins with a pI of 4.75‐6.25 and anti‐G3 serum labels proteins with a pI of 4.7‐6.6. Analyses of cells with and without trichocysts allow identification of possible precursors between 41 and 47 kDa. Some are in the same pI range as their putative products, but others, labeled by anti‐G3 serum, are less acidic than most of their mature products. Polyclonal antibodies Secretory proteins Secretory vesicles Immunoblots Immunofluorescence Immunogold labeling Dewey 590 44 Département de génétique et évolution 162 false The Journal of eukaryotic microbiology 40 1 bd69a7f2-a419-4d56-8d3c-dfc0c0b551ce Article (Published version) Restricted Eperon_JEM_19934081.pdf 7796063 application/pdf 7f6489aab9d7f8b96031ea16c2fb2eb41936f5b39057b5f2ee0a74f727e6817d restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Immunological Characterization of Trichocyst Proteins in the Ciliate Pseudomicrothorax dubius	The Journal of eukaryotic microbiology			1993	57	0

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Vigues, Bernard Patrick