Targeting Waddlia chondrophila development cycle using genetic tools and metabolomics

Diplôme Thèse Targeting Waddlia chondrophila development cycle using genetic tools and metabolomics eng 2019-04-18 author Walter Chantal 794182 director Cuendet Licea Muriel 0000-0002-1523-1907 686755 Muriel Cuendet 10.13097/archive-ouverte/unige:119059 urn:nbn:ch:unige-1190597 Sc. 5337 All members of the Chlamydiae, including Waddlia chondrophila share the same unique intracellular development cycle, which is the focus of this work in the search for anti-chlamydial targets and drugs. Strategies using high throughput screening, genetic complementation, and metabolomics were explored to investigate and try to block the W. chondrophila development cycle. First, a generic high throughput screening procedure was developed in a heterologous system, Escherichia coli, to search for inhibitors of chlamydial transcription factors. Then, the function of DksA, a transcription factor, was investigated by genetic complementation of E. coli and Pseudomonas aeruginosa mutants. Finally, metabolomics was adopted to observe changes in metabolites associated with progress through the chlamydial developmental cycle. It revealed the presence of putative novel metabolites differentially expressed in the various stages. Altogether, the results of this work present putative new therapeutic approaches to understand and circumvent the infection process of this pathogen. Dewey 615 31 Section des sciences pharmaceutiques 17 false 884 Pharmacognosie 4a0d9c58-18c2-424d-9ea2-6ce5a5675d86 Thesis Public 20190123_THESISofChantalWalter2018-final.pdf 5247020 application/pdf Private 2020-05-18 594cd0d3dec44946b6fd0af9f8f4f1cc2f5fe8050ca85c34a64124747df49040 openAccess Faculté des sciences / Section des sciences pharmaceutiques

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Targeting Waddlia chondrophila development cycle using genetic tools and metabolomics				2019	717	496

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Walter, Chantal