Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

Article Article scientifique Article Cell-free reconstitution reveals centriole cartwheel assembly mechanisms eng 2017-03-23 2017-03-23 author Guichard Paul 845268 Département de biologie moléculaire et cellulaire (MOCEL) author Hamel Virginie 846734 Département de biologie moléculaire et cellulaire (MOCEL) author Le Guennec Maeva 847004 author Banterle N. author Iacovache I. author Nemčíková V. author Flückiger I. author Goldie K. N. author Stahlberg H. 0000-0002-1185-4592 author Levy David 79041 author Zuber Benjamin 0000-0001-7725-5579 809179 author Giarre Gonczy Marianna Francesca P. 31539 1-9 14813 10.1038/ncomms14813 28332496 2041-1723 PMC5376648 https://www.nature.com/articles/ncomms14813 <p>How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-fold symmetrical cartwheel structure typically ∼100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 proteins. Whether nine-fold symmetrical ring-like assemblies of SAS-6 proteins harbour more peripheral cartwheel elements is unclear. Furthermore, the mechanisms governing ring stacking are not known. Here we develop a cell-free reconstitution system for core cartwheel assembly. Using cryo-electron tomography, we uncover that the Chlamydomonas reinhardtii proteins CrSAS-6 and Bld10p together drive assembly of the core cartwheel. Moreover, we discover that CrSAS-6 possesses autonomous properties that ensure self-organized ring stacking. Mathematical fitting of reconstituted cartwheel height distribution suggests a mechanism whereby preferential addition of pairs of SAS-6 rings governs cartwheel growth. In conclusion, we have developed a cell-free reconstitution system that reveals fundamental assembly principles at the root of centriole biogenesis.</p> Dewey 570 Swiss National Science Foundation Time-resolved structural study of calcium-dependent membrane fusion 163761 CH 46 Département de biologie cellulaire 165 false Nature communications 8 1 44284adb-b596-43ee-9cc0-c31eb0d27b26 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 2826446 application/pdf CC BY-4.0 18b26343e9e997be2aa72c466ac0dd8e1297259d90faa2f91a59bc03cffeedff openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Cell-free reconstitution reveals centriole cartwheel assembly mechanisms	Nature communications			2017	102	71

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Levy, David