On start and pause of replication fork

Diplôme Thèse On start and pause of replication fork eng 2018-09-04 author Shyian Maksym 786476 director Shore David M. 102587 10.13097/archive-ouverte/unige:110396 urn:nbn:ch:unige-1103966 Sc. 5255 In my PhD thesis I describe the work I've done with my colleagues in Professor David Shore's Lab that led to the next 3 main conclusions. (1) Conserved protein Rif1 is a negative regulator of DNA replication initiation in budding yeast. Rif1 counteracts DDK-dependent activatory phosphorylation at Mcm4 and Sld3 replication initiation proteins by recruiting protein phosphatase PP1 (ScGlc7) through its RVxF/SILK motifs. (2) Replication-restraining activity of Rif1 is important for the maintenance of genome integrity. Cells lacking Rif1 have elevated origin activation in early S phase at ribosomal RNA gene tandem repeat array (rDNA), which leads to destabilization of the array and dependency on DSB repair/fork maintenance factors MRX and Mms22-Ctf4 for survival. (3) Replisome pausing factors Tof1-Csm3 promote pausing at RFB independently of replisome accessory helicase Rrm3. Replication fork pausing at proteinaceous barriers (RFBs) depends on DNA lagging strand synthesis machinery and topoisomerases I and II. Dewey 570 43 Département de biologie moléculaire 161 false 813 Groupe Shore 15d0b765-a267-4fa5-b47d-c1b488ec9838 Thesis Public PhD_thesis-Maksym_SHYIAN-FINAL.pdf 13035986 application/pdf Private 2020-10-23 09a18a0716b72ef63eed27a04f63a115386f9bbe25d4f5fc3f3a92e1913476ca openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Shyian, Maksym