miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation

Article Article scientifique Article miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation eng 2022-02-15 author Tomasello Ugo 835285 author Klingler Esther 831648 author Niquille Mathieu 758199 author Mule Nandkishor Kisanrao 0000-0001-8429-2490 911317 author Santinha Antonio J author De Vevey Laura 781889 author Prados Julien 0000-0002-8546-241X 561771 author Platt Randall J author Borrell Victor 0000-0002-7833-3978 author Jabaudon Denis 0000-0003-2438-4769 682473 author Dayer Alexandre 84495 110381 10.1016/j.celrep.2022.110381 35172154 2211-1247 PMC8864305 Cortical expansion in primate brains relies on enlargement of germinal zones during a prolonged developmental period. Although most mammals have two cortical germinal zones, the ventricular zone (VZ) and subventricular zone (SVZ), gyrencephalic species display an additional germinal zone, the outer subventricular zone (oSVZ), which increases the number and diversity of neurons generated during corticogenesis. How the oSVZ emerged during evolution is poorly understood, but recent studies suggest a role for non-coding RNAs, which allow tight genetic program regulation during development. Here, using in vivo functional genetics, single-cell RNA sequencing, live imaging, and electrophysiology to assess progenitor and neuronal properties in mice, we identify two oSVZ-expressed microRNAs (miRNAs), miR-137 and miR-122, which regulate key cellular features of cortical expansion. miR-137 promotes basal progenitor self-replication and superficial layer neuron fate, whereas miR-122 decreases the pace of neuronal differentiation. These findings support a cell-type-specific role of miRNA-mediated gene expression in cortical expansion. Basal progenitors Cortex Evolution MicroRNA Neurogenesis Cell Proliferation / genetics MicroRNAs / genetics Neurogenesis / genetics Dewey 616.8 Dewey 616.89 Swiss National Science Foundation NCCR SYNAPSY: The synaptic bases of mental diseases (phase II) CH Programmes 53 Département de neurosciences fondamentales 21A 61 Département des neurosciences cliniques 226 60 Département de psychiatrie 225 false 540 Neurobiologie développementale et plasticité 880 535 Groupe Dayer Alexandre (Formation du circuit cortical) 875 0a6434c9-7972-44ea-8708-82e501660dc7 Biomedical Data Science Facility Cell reports 38 7 b1a006b0-38c2-4a02-a4cb-aedc4cdb9b0e Article (Published version) Public 1-s2.0-S2211124722001024-main.pdf 9485439 application/pdf CC BY-4.0 a236b83c-4e47-4357-92aa-cdbf75e9d0c2 Appendix Public gr5.jpg 267226 image/jpeg aaa9b8be-0541-40ae-9a20-d76cd7a214e0 Appendix Public gr3.jpg 332089 image/jpeg 364ac6d5-04c4-47f7-bc84-d976002621b9 Appendix Public gr4.jpg 419621 image/jpeg b7d9849a-cc51-47a4-90dd-35cf754d3784 Appendix Public gr2.jpg 362181 image/jpeg 2bdebe92-ab8a-47f5-9378-b72018cf6487 Appendix Public mmc1.pdf 11710881 application/pdf c32563c7-a2f1-4726-b6d1-243ddec41487 Appendix Public gr1.jpg 381833 image/jpeg 4d724587-ca82-414d-a205-c031a48244ed Appendix Public mmc2.pdf 22538092 application/pdf 08be1503-45ac-4f03-8414-6f8d0bbdc54a Appendix Public fx1.jpg 144675 image/jpeg Vignette Public 1-s2.0-S2211124722001024-fx1.jpg 78177 image/jpeg openAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques Faculté de médecine / Section de médecine clinique / Département de psychiatrie

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miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation	Cell reports			2022	342	305

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