Studies on immunoglobulins of Xenopus borealis, Xenopus clivii and Xenopus muelleri

Article Article scientifique Article Studies on immunoglobulins of Xenopus borealis, Xenopus clivii and Xenopus muelleri eng 2005-04-25 1976-01 author Fischberg Irandokht 25077 author Brandt Daniel author Bossus André 778944 author Michea Mehrbanou 25530 107-115 10.1002/jez.1401950110 815509 0022-104X https://onlinelibrary.wiley.com/doi/10.1002/jez.1401950110 Following immunization with human IgG three species of anuran amphibians, Xenopus borealis, Xenopus clivii and Xenopus muelleri, were found to synthesize two molecular populations of antibodies associated with 19S and 7S fractions of the sera. These antibodies, designated high (HMW) and low (LMW) molecular weight immunoglobulins, were isolated and their constituent heavy (H) and light (L) polypeptide chains separated following extensive reduction and alkylation in a relative yield of about 70% and 30% respectively. The molecular weights of H and L chains of the three species were determined by SDS-acrylamide gel electrophoresis. The L chains from both molecules in the three species had a molecular weight of about 26,000. The H chains of the HMW and LMW immunoglobulins had a molecular weight of about 73,000 and 63,000 respectively. The two populations of immunoglobulins were analysed in immunodiffusion plates using rabbit antisera to LMW immunoglobulins. The HMW immunoglobulins were found to be antigenically deficient with respect to LMW immunoglobulins. From these results it was judged that the two populations in each species belonged to distinct classes. The antigenicities of the LMW and HMW immunoglobulins of different species within the genus Xenopus were compared. Travaux du Département de biologie animale ; vol. 22, no 646 Dewey 590 44 Département de génétique et évolution 162 false The journal of experimental zoology 195 1 76d3cb54-8465-4439-917e-c1ec183291fa Article (Published version) Restricted Hadji_JEZ_1976195107.pdf 4654895 application/pdf c64c6c1045bd9830b9252b1e0550d58b3d8eacc499817458500bbb90478a16df restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Studies on immunoglobulins of Xenopus borealis, Xenopus clivii and Xenopus muelleri	The journal of experimental zoology			1976	1	0

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Bossus, André